药学毕业微波辅助提取黄芪多糖及含量测定.docxVIP

微波辅助提取黄芹多糖及含量测定 作者：龚苏晓，高展，张铁军，杨俊红，邸倩倩 □ □【摘要】 目的以微波从黄罠中提取多糖,并测定其含量。方法 运用微波技术提取黄罠多糖，采用正交设计法优选苯酚-浓硫酸法显 色条件，并测定其多糖含量。结果苯酚-浓硫酸法的最佳显色条件为 5%苯酚溶液的用量为1 ml，浓硫酸用量为5 ml冰浴温度为100°C , 水浴时间为15 min ;微波辅助提取黄罠多糖的最佳功率和时间为 900 W和30 mino结论微波辅助提取黄罠多糖，反应速度加快,实 验结果令人满意。 □【关键词】黄罠多糖苯酚■硫酸法微波提E □ □ Abstract:ObjectiveTo extract polysaccharides from Radix Astragali, with the microwave technology and determine its con tent. MethodsThe polysaccharides from Radix Astragali, were obtained by microwave technology. The way of coloration in the process of determining the content of polysaccharides from Radix Astragali was optimi