畜禽肌肉中肌苷酸测定的高效毛细管电泳法研究-食品科学.PDF

180 2009, Vol. 30, No. 06 食品科学 ※分析检测 1 1, 2 2 马 旺 ，蔡海莹 * ，檀华蓉 ，王艳红 (1.安徽农业大学动物科技学院，安徽合肥 230036 ；2.安徽农业大学生物技术中心，安徽合肥 230036) ：本实验对毛细管电泳(CE)测定畜禽肌肉中肌苷酸含量方法的建立进行了研究。采用P/ACETM MDQ 高效毛 细管电泳仪，电泳缓冲液为20mmol/L 磷酸氢二钾- 四硼酸钠，pH6.5 ，电泳温度为25 ℃，电压为22kV ，紫外检 测波长为248nm 。结果表明，肌肉中肌苷酸含量测定结果相对标准偏差为1.72%，后加标准品和前加标准品回收 率分别为80.91%～84.14% 和79.03%～82.41% 。该方法方便、快捷，对畜禽肌肉风味物质肌苷酸含量测定准确度 良好，是值得推广的一种新方法。 ：毛细管电泳；肌苷酸；肌肉 Determination of Inosinic Acid in Animal Muscles by High Performance Capillary Electrophoresis 1 1, 2 2 MA Wang ，CAI Hai-ying *，TAN Hua-rong ，WANG Yan-hong (1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China； 2. Biotechnology Center, Anhui Agricultural University, Heifei 230036, China) Abstract：The aim of this study was to set up a capillary electrophoresis method for determining inosinic acid in animal muscles. A P/ACETM MDQ high performance capillary electriphoresis (HPCE) instrument was adopted. The buffer, pH value, temperature, voltage and UV detection wavelength of HPCE were 20 mmol/L K2HPO4-NaB4O7 ·10H2O, 6.5, 25 ℃, 22 kV and 248 nm, respectively. The results showed that the relative standard deviation of inosinic acid in animal muscle is 1.72% and its recoveries of retro-plus and pro-plus standard preparations are 80.91% to 84.14% and 79.03% to 82.41%. This new method is convenient, rapid and accurate for assaying inosinic acid in muscle flavor substance and deserved to be spread. Key words ：capillary electrophoresis ；inosinic acid ；muscle 中图分类号：TS207.3 文献标识码：A 文章编号：1002-6630(2009)06-01