五味子提取物对大鼠肝CYP3A酶的双重作用Dualeffectsofextractof.PDF

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五味子提取物对大鼠肝CYP3A酶的双重作用Dualeffectsofextractof.PDF

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1194 药学学报 Acta Pharmaceutica Sinica 2010, 45 (9) : 1194 1198 ·研究简报· 五味子提取物对大鼠肝CYP3A 酶的双重作用 # * 倩, 吴育晶, 程能能 , 李雅琳, 王永铭 (复旦大学药学院药理学教研室, 上海 201203) 关键词: 五味子; 五味子甲素; 五味子乙素; 五味子醇乙; 南五味子素; CYP3A; 肝微粒体; 原代肝细胞中图分类号: R285 文献标识码:A 文章编号: 05 13-4870 (2010) 09-1194-05 Dual effects of extract of Schisandra chinensis Baill on rat hepatic CYP3A # * CHEN Qian, WU Yu-ji ng , CHENG Neng-neng , LI Ya-lin, WANG Yong-ming (Depa rtment of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China) Abstract: This study is to investigate the effects of aqueous extract of Schisandra chinensis Baill (WWZ), kadsurin, schisandrin A, schisandrin B and schisandrol B on rat hepatic CYP3A. Rats received a daily gavage of aqueous extract of WWZ for different times. The livers were harvested after gavage and subj ected to microsome preparation. Microsomal CYP3A activity was determined by measuring the amount of the metabolite of testosterone (6 -hydroxytestosterone) with HPLC. Aqueous extract of WWZ, kadsurin and schisandrin A were incubated with microsomes obtained from rat. Microsomal CYP3A activity was determined by HPLC. Primary hepatocytes were separated and extracted from rat, then were treated with aqueous extract of WWZ, schisandrin A, schisandrin B and schisandrol B. Then, the expression of CYP3A 1 mRNA was analyzed by RT-PCR. As for the in vivo assay, aqueous