显微镜检查与干化学试纸条检测尿红、白细胞临床应用探讨.doc

PAGE PAGE 1 显微镜检查与干化学试纸条检测尿红、白细胞临床应用探讨 【摘要】目的：探讨用3种显微镜检查法与2种干化学试纸条检测尿红，白细胞的应用价值。方法：按NCCLSLiteratureGP16－A的要求收集1236份临床尿液标本，同时用DiaSysR/S2003尿液分析工作站法、非染色尿沉渣镜检法、过筛法和MultistixTM10SG,Combur10Testreg;M两种干化学试纸条法进行分析。结果：3种镜检方法与2种干化学试纸条法符合率约在50.0％左右，红细胞与隐血比较，符合率仅为46.2％～55.2％；白细胞与白细胞酯酶比较，符合仅为45.0％～53.3％。MultistixTM10SG试纸条检测隐血，白细胞酯酶的假阳性率高于Combur10Testreg;M，假阴性率低于Combur10Testreg;M。结论：干化学法检测尿红、白细胞有相当高假阳性率和假阴性率；进行尿液分析时应按标准化的要求进行外观/理学，化学，显微镜检查。 【关键词】尿沉渣显微镜检查；尿干化学检查；红细胞；白细胞 Theinvestigationoftheclinicalapplicationontheexaminationsoftheurineerythrocytesandleukocyteswiththeexaminationofmicroscopeandreagentstripe. Zhangxiaokun,Xujianbang,Liuganhui,Guandaguang,Fanguoyao,Caoyuming,Departmentofclinicallaboratory,LiwanHospital,GuangzhouMedicalcollege,Guangzhou510170,China;2.ThesecondAffiliatedHospital,GuangzhouMedicalCollege,Guangzhou510260,China. [Abstract]ObjectiveToinvestigatetheapplicationworthamongthreemethodsofmicroscopeandtwomethodsofreagentstripe.Methodscollecting1236casesofclinicalurinesamplesundertherequirementofNCCLSLiteratureGP16-A,AnalysisurineRBCandurineWBCwiththeDiasysR/S2003urinesedimentsanalysisworkstation(Diasys),non-stainmiscroscopicexamination(NSME),Filtrationexamination(FE)andtwomethodsofreagentstripeofMultistixTM10SGandcombur10Testreg;M.ResultThreemethodsofMicroscopeexminationandtwomethodsofreagentstripeshowtheconformabilityas50.0%;RBCandBLO46.2%-55.2%;WBCandLEU45.0%-53.3%.MultistixTM10SGreagentstripehasahighrateoferrordiagnostie(RED)andhasalowlateoflostdiagnostic(RLD)thancombur10Testreg;MontheBLOandLEUresults.ConclusionreagentstriphasahighrateofRLDandREDontheexaminationoftheurineRBCandurineWBC.thestandardizationandregulationoftheexaminationofappearance,physics,chemistryandmincroscopewassuggestedontheurineanalysis. [keyword]urinesedimentsworkstation;urinereagentstrips;erythrocytes;leukocytes 自1956年，在DrAlfedHelenFree领导下，开创了ldquo;浸与读rdquo;（dipandread）干化学法的新纪元，经近半个世纪发展，多联式试纸条相继问世配合尿液分析仪广泛进入实验室，为尿液化学成份检查提供快捷、可靠、客观的结果，成为尿液分析重要的组成部分。然而，因尿干化学检查法在检测原理上有一定的局限性、部分使用者对仪器缺