胃蛋白酶与对磺酸基杯芳烃相互作用的研究分析A.docVIP

个人收集整理 仅供参考学习 个人收集整理 仅供参考学习 PAGE / NUMPAGES 个人收集整理 仅供参考学习 胃蛋白酶与对磺酸基杯芳烃相互作用地研究 刘睿*1,3，周兵川2，张军伟1，靳朝喜1，林琳3（1.洛阳市环境监测站，河南 洛阳 471000；2.嵩县环境监测站，河南 嵩县471400；3.郑州大学化学系，河南 郑州 450052） 摘 要：采用荧光光谱法和紫外可见分光光度法研究了胃蛋白酶与对磺酸基杯芳烃和对磺酸基杯芳烃相互作用时光谱性质地变化,发现两种对磺酸基杯芳烃对胃蛋白酶地荧光均有猝灭作用，并根据Stern-Volmer方程及紫外－可见吸收光谱得出猝灭机理均为静态猝灭.根据反应地热力学参数得出对磺酸基杯芳烃对胃蛋白酶地作用力均属于静电作用力，并依据F?rster无辐射能量转移理论得出荧光给体（胃蛋白酶）－受体（对磺酸基杯芳烃）间地距离r.用同步荧光光谱技术考察了两种对磺酸基杯芳烃对胃蛋白酶构象地影响.b5E2RGbCAP 关键词：胃蛋白酶；对磺酸基杯芳烃；荧光光谱法；相互作用 中图分类号：O 657.3 文献标识码：A 文章编号： Study on the interaction between 4-Sulfonic calixarene and pepsin.p1EanqFDPw LIU Rui*1,3,ZHOU Bing-chuan2,ZHANG Jun-wei1,JIN Chao-xi1,LIN Lin3, (1.Luoyang Environmental Monitoring Station,Luoyang471000,China;2.Songxian Environmental Monitoring Station,Luoyang 471000,China;3.Department of Chemistry, Zhengzhou University, Zhengzhou 450052,China)DXDiTa9E3d Abstract: Spectrum Properties study on the interaction of 4-Sulfonic calixarene and 4-Sulfonic calixarene with pepsin were investigated by fluorescence spectroscopy and UV-vis absorption spectroscopy. We found that the two 4-Sulfonic calixarene could quench the fluorescence of pepsin. Based on the Stern-Volmer equation and UV-vis spectrum, the quenching mechanisms were all static quenching. The thermodynamic parameters were calculated, and indicated that the electrostatic forces played a major role in the interaction between 4-Sulfonic calixarene and pepsin. The distance r between the donor (pepsin) and the acceptor (4-Sulfonic calixarene) were determined according to F?rster non-radiative energy transfer theory. The effect of 4-Sulfonic calixarene on the conformation of pepsin was investigated by synchronous fluorescence spectrum.RTCrpUDGiT Key words: Pepsin; 4-Sulfonic calixarene; Fluorescence spectroscopy; Interaction5PCzVD7HxA 胃蛋白酶（Pepsin）是胃液中主要地蛋白酶，属于水解蛋白酶中地羧基蛋白酶，因含有酪氨酸、色氨酸和苯丙氨酸残基而能发射荧光.胃蛋白酶自 Northrop 于1929年将其结晶以来[1]就在生命科学[2]、食品科学[3]、分析化学[4]等领域得到了广泛地研究，至今仍是人们关注地研究热点之一.jLBHrnAILg 杯芳烃是一类由苯酚和甲醛缩合而成地大环化合物,由于具有与环糊精和冠醚相似地性质而被誉为