氯化锂诱变赤灵芝原生质体选育高产三萜类菌株-食品科学.PDF

120 2011, Vol. 32, No. 01 食品科学 ※生物工程 董玉玮，苗敬芝，吕兆启，曹泽虹，高明侠 (徐州工程学院食品(生物)工程学院，江苏 徐州 221008) 采用化学诱变剂氯化锂诱变赤灵芝原生质体，对高产三萜类菌株进行筛选。结果表明：经 0.3% 氯化锂 -2 诱变后，原生质体致死率为 72.8% ，胞内、胞外三萜类产量达到最高，分别为55.33mg/g 和 14.88 ×10 mg/mL ， 比原菌株分别提高了461.72% 和 136.57%。第2 代、第 3 代诱变菌株比原菌株胞内三萜类产量分别提高了 483.05% 和 496.04% ，胞外三萜类产量分别提高了 158.66% 和 171.38%，诱变菌株遗传稳定性良好。 赤灵芝；原生质体；氯化锂；诱变；三萜类 Screening and Breeding of High-yield Triterpenes-producing Ganoderma lucidum Mutants Induced by Lithium Chloride .. DONG Yu-wei ，MIAO Jing-zhi ，LU Zhao-qi ，CAO Ze-hong ，GAO Ming-xia (College of Food (Biology) Engineering, Xuzhou Institute of Technology, Xuzhou 221008, China) Abstract ：Lithium chloride was used to induce the mutation of Ganoderma lucidum protoplasts in order to obtain high-yield triterpenes-producing mutants. The results showed that the optimal mutation condition was treatment with 0.3% lithium chloride, resulting in 72.8% fatality rate. The first generation of the mutant obtained exhibited intra- and extracellular triterpenes -2 mg/mL, higher than those of the original strain by 461.72% and 136.57%, respectively. In yields of 55.33 mg/g and 14.88 ×10 comparison with the original strain, the second and third generations exhibited increases by 483.05% and 496.04% in intracellular triterpenes yield and by 158.66% and 171.38% in extracellular triterpenes yield. Therefore, it can be concluded that the mutant induced by 0.3% lithium chloride is genetically stable. Key words ：Ganoderma lucidum；protoplasts ；lithium chloride ；mutagenesis ；trit