手术诱导的小鼠骨关节炎模型中关节软骨MMP-第三军医大学学报.DOCVIP

手术诱导的小鼠骨关节炎模型中关节软骨MMP-13表达上调 唐浚洲，苏 楠，周思儒，李晓刚，谢杨丽，陈 林 （400042 重庆，第三军医大学大坪医院野战外科研究所创伤实验室，骨代谢修复中心，创伤、烧伤与复合伤国家重点实验室 ） [摘要] 目的 观察小鼠骨关节炎模型中关节软骨组织中基质金属蛋白酶13（matrix metalloproteinase 13，MMP-13）表达水平的变化，探讨其与关节损伤之间的关系。方法 在小鼠原代软骨细胞培养中用IL-1β诱导炎症细胞外环境，并采用RT-PCR检测MMP-13的表达水平。手术诱导不稳定的内侧半月板（destabilization of the medial meniscus，DMM）建立小鼠骨关节炎模型，观察关节软骨组织变化情况，并用免疫组化检测MMP-13在受损的关节软骨中的表达。结果 用IL-1β处理的原代软骨细胞中MMP-13的mRNA表达明显较高[（3.89±0.39） vs 1，P0.01]。DMM术后小鼠关节软骨出现骨关节炎表现，且术后10周小鼠关节损伤程度较术后5周小鼠严重。免疫组化结果显示术后5周小鼠软骨组织中MMP-13的表达高于对照组；术后10周软骨组织中MMP-13进一步增高。结论 在小鼠骨关节炎模型发病过程中，MMP-13作为一个具有软骨破坏性的标志分子在关节软骨中持续高表达，在骨关节炎发生过程中起重要作用。 [关键词] 骨关节炎；手术诱导；不稳定的内侧半月板；基质金属蛋白酶13；骨关节炎模型；小鼠 [中图法分类号] [文献标志码] A Increased expression level of MMP13 in articular cartilage in surgically induced osteoarthritis mouse mice Tang Junzhou, Su Nan, Zhou Siru, Li Xiaogang, Xie Yangli, Chen Lin （(State Key Laboratory of Trauma，, Burns and Combined Injury，, Center of Bone Metabolism and Repair，, Institute of Surgery Research，, Daping Hospital，, Third Military Medical University，, Chongqing，, 400042，, China）) [Abstract] Objective Extracelluar environment which surround articular chondrocytes changes in osteoarthritis. We undertook this study toTo determine investigate the change of matrix metalloproteinases 13 (expression level of MMP13) expression in the articular cartilage of osteoarthritis mice and investigate its correlation with articular injuryin murine osteoarthritis model. Methods Murine articular chondrocytes were isolated from neonatal mice and cultured primarily, and then treated with 10 ng/mL interleukin-1β (IL-1β) for 24 h. The mRNA expression of MMP13 was detected by Inflammation extracelluar environment was induced by interleukin-1β (IL-1β) in cultured primary murine articular chondrocytes and matrix metalloproteinases (MMPs) 13 messenger RNA (mRNA) expression was measured by quantitative real-time reverse transcriptase-polymerase chain reaction. A total of 30 C57BL/6J mice