对硫磷处理下果蝇细胞应激基因的高通量分析.pdf

Chinese Journal of Applied Entomology 2015, 52(2): 411418. DOI: 10.7679/j.issn.20951353.2015.045 * 对硫磷处理下果蝇细胞应激基因的高通量分析 ** *** 李 娜 陈 梓 刘 为 迟庆萍 胡俊丽 程罗根 210023 摘 要 【目的】 Drosophila melanogaster (William)S2 【方法】CCK-8 (RNA-Seq) PCR(qRT-PCR) 【结果】35 μg/mL 35 μg/mL 35 μg/mL 481 52 qRT-PCR 【结论】 关键词 qRT-PCR RNA-Seq (quantitative) analysis of the stress gene in Drosophila cells treated with parathion ** *** LI Na CHEN Zi LIU Wei CHI Qing-Ping HU Jun-Li CHENG Luo-Gen (School of Life Sciences, Nanjing Normal University, Nanjing 210023, China) Abstract [Objectives] To investigate stress gene expression, and quantitative changes in the expression of this gene, in Drosophila S2 cells treated with parathion, and provide a basis for further analysis of insect stress reactions and molecular toxicology. [Methods] The cytotoxicity and cell survival rate of Drosophila S2 cells were tested using CC-8 reagents and a cell count plate. Application of RNA-Seq and qRT-PCR to detect differentially expressed genes and quantitative changes in gene expression. [Results] Cells exposed to 35 μg/mL parathion had high survival rates. Analysis of changes in gene expression with RNA-Seq revealed that 48 1 genes were up-regulated and that 52 displayed significant changes. These included oxidative stress genes, detoxification genes an