柑桔溃疡病菌抗药突变体的生理特性研究.PDFVIP

植物病理学报 ( ) ( ) ACTA PHYTOPATHOLOGICA SINICA 　33 1 :6366 2003 柑桔溃疡病菌抗药突变体的生理特性研究 黄青春 , 周明国 , 叶钟音 (南京农业大学植物保护学院 , 南京 210095) ( ) - 6 摘要 : 经药剂驯化和紫外光诱导双重作用可获得柑桔溃疡病菌抗拌种灵突变体 XcR ,病原菌抗药突变频率低于 10 。XcR 的 生长速率与敏感菌株基本一致 ,对萎锈灵和叶枯唑具有潜在的正交互抗性 ,与浸种灵没有交互抗性。XcR 胞外产物和胞外水解 酶活性一般多于或高于敏感菌株 ,其中以淀粉酶活性变化最为明显。XcR 琥珀酸脱氢酶活性显著降低 ,并对拌种灵的敏感性亦 降低。XcR 能够引起烟草的过敏性反应 ,但失去对寄主的致病性。XcR 的性质表明 ,柑桔溃疡病菌对拌种灵产生抗药性的风险 较低 ,病原菌获得抗药性状的同时可能导致其在田间的适合度下降。琥珀酸脱氢酶的活性可作为柑桔溃疡病菌抗药性的标记。 关键词 : 柑桔溃疡病菌 ; 抗药突变体 ; 拌种灵 Physiological characters of the resistant mutant of Xanthomonas citri to amicarthiazol 　 (College of Plant Protection , Nanjing Agricultural University , Nanjing 210095 ,China) HUANG Qingchun , ZHOU Mingguo , YE Zhongyin 　 Abstract : The resistant mutant of X . citri (XcR) to amicarthiazol was obtained by dual treatments with amicarthiazol ta - 6 ming and ultraviolet irradiation with a frequency lower than 10 . XcR possessed the similar growth velocity as the sensitive isolate ( XcS) , and had positive cross resistance to carboxin and bismerthiazol fungicides , but not to diisothiocyana tomethane. Investigation on physiological characters showed that XcR could secrete extracellular enzymes , such as amylase , pectase , cellulosase , proteinase and lipase. The bioactivities of these enzymes were usually higher than that of XcS , espe cially for amylase. The activity of succinate dehydrogenase of XcR was obviously reduced and not sensitive to the inhib