微柱高效液相色谱法测定杨梅黄酮类物质.pdf

2004 67 w94 1,2 2 2 2 2 1 2 ( 653100 650091) 2.0min 97 103%1.6%2.2% The Determination of Myrica Rubra Flavonoid by Microcolumn High Performance Liquid Chromatography Hu Qiufen 1,2, Yang Yaling 1, Huang Zhangjie 2, Yang Guangyu 2, Yin Jiayuan 2 1 2 ( Department of Chemistry, Yuxi Teacher’s College, Yuxi 653100; Department of Chemistry, Yunnan University, Kunming 650091) Abstract A microcolumn high performance liquid chromatography (HPLC) method for the determination of flavonoid in myrica rubra leaf and bark was studied. The flavonoid was separated on a Waters XterraTM RP18 (1.0×30mm, 2.5µm) chromatographic column, with methanol and 0.05mol/L potassium dihydrogen phosphate buffer solution as mobile phase at a flow-rate of 0.2mL/min, and monitored with the photodiode array detector at 360nm. The recoveries of the flavonoid are 97%103%, and relative standard deviations are 1.6%2.2%. Key words Microcolumn high performance liquid chromatography, Solid phase extraction, Flavonoid, Myrica rubra [1∼4] [5] 2.0min 1 1.1 2690 ( ) 996 Millennium32 Waters Allaince 30 E-mail: huqiufena@163.com (0211143)(020024) 2003-11-30 2004-03-15 2004 67 w94 Fisher Milli-Q50( -1 Millipore ) ≥18Ω·cm Fluka HPLC -3-O-β-D- -3-O-α-L- 3- O-β-D--3-O-α-L- Fluka