陆地棉抗旱性与SSR分子标记的关联分析-中国棉花杂志社.PDFVIP

棉花 学报 Cotton Science 20 17, 29(3): 241―252 陆地棉抗旱性与SSR 分子标记的关联分析 桑晓慧袁赵云雷袁王红梅* 袁陈伟袁龚海燕袁赵佩袁崔艳利 中国农业科学院棉花研究所/ 棉花生物学国家重点实验室袁河南安阳455000 冤 摘要院揖目的铱了解陆地棉抗旱性和寻找与陆地棉抗旱性相关的分子标记袁挖掘与抗旱性状相关的优异等位变 异遥 揖方法铱以191 份陆地棉栽培种组成的自然群体为材料袁在萌发期采用质量分数为15%的PEG6000 竖直滤 纸法对191 份材料进行胁迫处理袁测定其发芽率遥根据隶属函数值对萌发期抗旱性划分抗旱级别遥利用关联分 析的方法以74 对SSR 引物对该自然群体进行基因组变异扫描遥在利用Structure 2.3.4 分析该自然群体的遗传 结构的基础上袁使用Tassel 2.0 的GLM 模型将基因型数据与萌发期抗旱性状进行关联分析袁寻找与抗旱性状 相关的分子标记遥 揖结果铱筛选出强抗旱材料46 份袁中等抗旱材料59 份袁干旱敏感材料86 份曰群体结构分析将 191 份材料划分为2 个亚群袁分别包含 123 份和68 份材料曰通过关联分析获得与陆地棉萌发期抗旱性显著相 关的分子标记 15 个袁各位点对表型变异解释率为2.2％～7.49 ％遥 揖结论铱本研究筛选出强抗旱材料46 份袁获 得与萌发期抗旱性相关的分子标记15 个袁可供棉花抗旱标记辅助选择育种利用遥 关键词院陆地棉曰SSR 曰关联分析曰抗旱性 中图分类号院S562.035 文献标志码院A 文章编号院1002-7807 2017 冤03-0241-12 院10.11963/ 1002-7807.sxhwhm.20 170407 * Sang Xiaohui, Zhao Yunlei, Wang Hongmei , Chen Wei, Gong Haiyan, Zhao Pei, Cui Yanli ( 455000, ) [Objective] The aims of this study are to detect drought tolerance of upland cotton ( L.), to find the molecular markers associated with the drought tolerance traits, and to excavate elite allelic variation associated with drought re- sistance traits. [Method] We selected 19 1 cultivars (lines) as experimental materials. The germination rates during sprouting peri- od were evaluated in 15% (mass fraction) PEG6000 stress. Drought tolerance was classified according to the membership func- tion values to drought resistance at sprouting period. A total of 74 pairs of simple sequence repeat (SSR) primers were employed to detect the genomic variation of the natural population. Based on the analysis of the genetic structure of the natural population using Structure 2.3.4, the GLM model of Tassel 2.0 was used to correlate the genotype data with the drought tolerance traits at germination stage to fin