高效液相色谱法测定大鼠血浆和子宫中黄体酮及其-中国科技论文在线.PDFVIP

药学学报Acta Pharmaceutica Sinica 2004，39(8)：613—617 ·6l3· 高效液相色谱法测定大鼠血浆和子宫中黄体酮及其代谢物的浓度 张立雯，晁若冰 (四川大学华西药学院，四川 成都610041) 摘要：目的 建立高效液相色谱法测定大鼠血浆和子宫样品中的黄体酮及其代谢物20 一羟基黄体酮，并研究大 鼠肌肉注射黄体酮后血浆和子宫中的药物代谢动力学。方法 样品经液一液萃取后，以乙腈一水(60：40，pH 4．0)为流 动相，用ODS柱进行分离，240 nm检测。以18一甲基炔诺酮为内标。结果 血浆中黄体酮C…为(508±62) g·L～，r，…为(3．2±0．4)h，T ，!( )为(10±4)h，AUC。I48 h为(5 886±1 573)I．zg-L～·h，子宫中黄体酮r厂m 为 (5．2±1．1)h，C…(1．7±1．1)I．zg·g～。20 一羟基黄体酮具有与黄体酮相似的r厂m 。结论 该方法简便、准确，可 同时测定黄体酮和代谢物，适用于黄体酮及其代谢物20 一羟基黄体酮的药代动力学研究。 关键词：黄体酮；20 一羟基黄体酮；高效液相色谱法；血浆；子宫 中图分类号：R917．101 文献标识码：A 文章编号：0513—4870(2004)08—0613—05 Determination of progesterone and its main metabolite in rat plasma and uterus using HPLC ZHANG Li—wen，CHAO Ruo—bing (West China School of Pharmacy，Sichuan University，Chengdu 610041，China) Abstract：Aim To quantify progesterone(P)and one of its metabolites 20or—hydroxy一4一pregnen一3一 one(2Oct—OHP)in rat plasma and uterus after im administration of progesterone．Methods Plasma and uterus samples were prepared by liquid·-liquid extraction and separated through Shimadzu VP·-ODS column (150 mm x 4．6 mm ID，5 Ixm)．The mobile phase consisted of acetonitrile and water(60：40，adjusted to pH 4．0 with phosphoric acid)．The detector was set at 240 nm．Norgestrel was used as the internal standard．Results C。 ofP in plasma was(508±62)Ixg·L～，T…was(3．2±0．4)h，Tl／2(k )was (10±4)h and mean AUC0堋h was(5 886±1 573)Ixg·L～·h．The maximum concentration of P in uterus was(1．7±1．1)Ixg。g一 and the peak time was(5．2±1．1)h．20 —OHP showed a similar T with P．Conclusion The method iS accurate and convenient．It can be used to determine P and its main metabolite 20 一OHP simultaneously for studying their preclinical