适形放疗作用胃癌的蛋白表达谱变化及意义-第三军医大学学报.DOCVIP

适形放疗作用胃癌的蛋白表达谱变化及意义 通讯作者简介及联系方式： 蒋明东：（1965- ），男，四川蓬溪人，副主任医师，生物医学工程硕士，研究高强度聚焦超声治疗肝癌安全性，肿瘤学博士，研究肝癌多药耐药的基因和药物逆转。主要从事临床肿瘤诊断和治疗工作，发表论文21篇。 Tel： (023Fax： (023E-mail： jmdxgh@163.com Address：重庆市第九人民医院肿瘤科 Postalcode: 400700 收稿日期： 修回日期： 基金项目：重庆市卫生局科研资助项目（07-2-219） 适形放疗作用胃癌的蛋白表达谱变化及意义 蒋明东1 向廷秀2 李少林3 仝蜀生4 李铁军4 赵瑜1 黄小波1 （1．重庆市第九人民医院肿瘤科，重庆北碚 400700，2．重庆医科大学附属第一医院实验研究中心，重庆渝中 400016， 3．重庆医科大学基础医学院放射教研室，重庆渝中 400016，4．重庆市第九人民医院普外科，重庆北碚 400700） The alterations and significances of protein expression profile in gastric cancer with conformal radiation therapy JIANG Ming-dong XIANG Ting-xiu LI Shao-lin TONG Shu-sheng LI Tie-jun ZHAO Yu HUANG Xiao-bo 1．Department of Oncology, The Ninth People Hospital of Chongqing, Chongqing Beibei, 400700 2．The Empirical Research Center of the Affiliated First Hospital of Medical University in Chongqing, Chongqing YuZhong, 400016 3．Teaching Laboratory of Radiation for College of Preclinical Medicine in Chongqing Medical University, Chongqing YuZhong, 400016 3．Department of Ordinary Surgery, The Ninth People Hospital of Chongqing, Chongqing Beibei, 400700 【Abstract】 AIM: To investigate the alterations and significances of protein expression profile in gastric cancer with conformal radiation therapy. METHODS: The pathologically-diagnosed gastric cancer patients were chosen randomly to perform conformal radiation therapy before the operation. And the non-conformal radiation treatment group was as the control. The tumor tissues were collected from these patients after operation both in the two groups. And then the alterations of protein expression profile were detected by 2-direction gel electrophoresis (2-D Gel) and MALDI-TOF-MS. RESULTS: The protein expression profile of tumor tissues from gastric cancer patients receiving conformal radiation therapy was obviously altered. And the expressions of VEGF, CerbB-2 and EGFR were reduced in this group compared with patients without conformal radiation therapy. CON