软枣猕猴桃多糖的分离与纯化及其分子质量的测定-食品科学.PDF

66 2012, Vol. 33, No. 15 食品科学 ※基础研究 潘 松，刘长江 * ，梁 爽 (沈阳农业大学食品学院，辽宁 沈阳 110866) 对通过水提醇沉法提取、Sevag 法脱蛋白得到的软枣猕猴桃多糖进行分离纯化，利用 DEAE- 纤维素 52 离 子交换层析对软枣猕猴桃多糖进行初步分离，得到4 种软枣猕猴桃多糖组分；利用葡聚糖凝胶对软枣猕猴桃多糖组 分进一步分离的最佳条件为葡聚糖凝胶型号为 Sephadex G-200、层析柱规格为D 1.1cm ×100cm、多糖质量浓度为 10g/L。以此条件分离，得到含量较高的软枣猕猴桃多糖- Ⅱ纯品。通过Sephadex G-200 凝胶柱层析鉴定软枣猕猴 桃多糖 - Ⅱ是均一的纯多糖，通过紫外光谱鉴定软枣猕猴桃多糖- Ⅱ不含核酸及蛋白。同时测得软枣猕猴桃多糖- Ⅱ 的分子质量为83733D 。 软枣猕猴桃；多糖；分离纯化；柱层析；紫外光谱；分子质量测定 Isolation, Purification and Molecular Weight Determination of Polysaccharides from Actinidia arguta Sieb. et Zucc. Fruits PAN Song ，LIU Chang-jiang* ，LIANG Shuang (College of Food Science, Shenyang Agricultural University, Shenyang 110866, China) Abstract ：Polysaccharides from Actinidia arguta Sieb. et Zucc. were extracted by hot-water extraction, ethanol precipitation, protein removal by the Sevag method and then purified by DEAE-cellulose-52 ion exchange column chromatography. Four polysaccharide fractions were obtained. The best purification of Fraction Ⅱ was achieved by chromatographic separa- tion on a Sephadex G-200 column (D 1.1 cm × 100 cm) at a sample concentration of 10 g/L. As a result, a homogenous pure polysaccharide was obtained. The results of UV spectroscopic analysis revealed that the purified polysaccharide contained neither proteins nor nucleic acids. Moreover, the molecular weight of the purified polysaccharide was measured to be 83733 D. Key words ：Actinidia arguta Sieb. et Zucc. ；polysaccharide ；isolation and purification ；column chromatography ； ultraviolet spectroscopy ；molecular weight determination 中图分类号：Q539 文献标识码：A 文章编号：1002-6630(