茶叶农药残留检测中咖啡因干扰的去除-食品科学.PDF

194 2009, Vol. 30, No. 12 食品科学 ※分析检测 茶叶农药残留检测中咖啡因干扰的去除 芮三亚，胡 奇，蔡潞莎，陈云雯 (南京市农产品质量监督检验站，江苏 南京 210036) 摘 要：用热水(45℃) ＋有机溶剂(二氯甲烷) 的提取法，结合Carbosil 小柱(用于有机磷农残)和Florisil 柱(用于菊酯 类农残)净化处理，除去茶叶中的咖啡因对农药残留检测的干扰；对茶叶中常检农药残留如拟除虫菊酯类农药(联苯 菊酯、甲氰菊酯、氰戊菊酯 、氯氰菊酯、溴氰菊酯等) 和有机磷农药( 滴滴畏、乐果、甲基对硫磷、喹硫磷、 三唑磷等)在三个加标水平 0.2 、0.8 、1.6mg/kg 上进行了方法的单实验室验证。结果表明：本前处理方法能有效去 除茶叶中咖啡因对农药残留检测的干扰；对上述有机磷农残各加标水平上的平均回收率在73%～112.9% 之间，RSD 8% ，最低检出限在0.002～0.02mg/kg ；对上述菊酯类农残各加标水平上的平均回收率在78.8%～105% 之间，RSD 9% ，最低检出限在0.001～0.006mg/kg ，均符合或优于我国和欧盟有关法规的规定。 关键词：茶叶；有机磷农药残留；菊酯残留；咖啡因 Elimination of Caffeine Disturbance to GC Determination of Pesticides Residues in Tea RUI San-ya ，HU Qi ，CAI Lu-sha ，CHEN Yun-wen (Nanjing Supervision and Inspection Station of Agricultural Products Quality, Nanjing 210036, China) 　　　　　　　 Abstract ：A pretreatment method of dichloromethane plus hot water （45 ℃）extraction followed by cleanup with Carbosil cartridge (for organophouros pesticides residues) or Florisil cartridge (for pyrethrosins residues) was developed for eliminating the disturbance of caffeine to the GC determination of pesticides residues in tea. A single laboratory validation was performed by spiking organophouros and pyrethrosins pesticides at three levels (0.2, 0.8, and 1.6 mg/kg, respectively). The results showed that the caffeine was effectively removed from tea extract by this method. The average recoveries for organophouros pesticidies were between 73% and 112.9%, the RSDs were less than 8%, and the limits of detection ranged between 0.02 and 0.002 mg/kg; for pyrethrosins, the average recoveries varied between 78.8% and 105%, the RSDs were below 9%, and the limits of detection ranged from 0.001 to 0.006 mg/kg. The accuracy, precisio