基于生物芯片的中药作用机理研究-机械电子工程专业论文.docxVIP

拜托■：媳天学学艇娩文 拜托■：媳天学学艇娩文 摘笙 摘 要 传统中药由予其作鞠枫理难以用现行璺￡物医攀理论解释，在围际社会上一直 没蠢获褥公众浆蛰遮诀爨裰接受。隧差缘热环保糕念豹发展，对予审药基鼹终矮 枫联的研农翻益褥剿人{f]骢莛视，褥基西旗片是一张潜在熬磷究势物搀疆枫理的 蠢力工兵。麓鳄将孛菸戆撬势与褒蕊辩学撬寒豹方法帮手毅蠢效燕羲缝台，已戏为 字装磺究领域躲主鼷发袋方怒之～。零文瓯陂曩予蕊镑穆溪辍理萋野究戆表达遴芯 片为辩象糙开研炎。 善先，缀合璺糖葱冀靛橇念发潮备方法，详继毒孪论7褥突孛药{窜雳擒懋鳃意 义，以及藻函芯片成用于中药机瑷研究韵现状与发展。 其次，辩基鞭袭这谱蕊冀的檄念、·捡测艨理殿其实验滚毽进行’r磷炎，簧在 确定了实验对象豹鏊础上进行了实验设计。通过实验，获得了结裂芯片及大燕的 实验数摇。 最爱，农辩蘩鼹芯冀熬数学分掇方法瀵稽了秘寒惹，逯爆其中熬手工分类法 越本次实狻数蕹滋褥了分橱，辩安验绩果迸稽了《纛缝分褫。 罨|手褒淡秘，螯嚣表遮灌茶冀栽昭舞效、攥确撼羲}f究串蕊律焉钒理；手工分类 法疑蒋快滚、童飘簸理文蘩数据的特点。 芙壤谶：巾蘸、终斌枫制、生魏拣麓、蒸瓣裳迷灌、数爨熊理、乎工分爽滚 西北T业大学学位论文 西北T业大学学位论文 ABSTRACT ABSTRACT In international society,traditional Chinese medicine(TCM)has not been widely identified and accepted by pubis due to the reason of difficulty in explaining the acting mechanism using the theory of modem biomedicine．Along with the development of the conception of green environmental protection，research for the acting mechanism of traditional Chinese medicine in gene level has increasingly attracted people’S attention．Biochip is a kind of potential and powerful tool to study the function mechanism of medicine．How to effectively combine the advantage of the traditional Chinese medieine and the methods and means of modern science and technology has already become the important issue in the TCM field．DNA microarrays for gene expression profiles for dmg function mechanism research has been explored in this paper． According to the concept ofthe biochip and its preparation method，the meaning of the acting mechanism study of TCM，the actuality and development of application of gene chip to TCM mechanism research，have been discussed． Then，the concept，measuring principal and experiment flow of the DNA microarrays for gene expression profiles were studied．On the basis of defining experimental subjects，the experiments have been designed． Finally,after studying the mathematical methods of anglicizing gene chip， experimental data were analyzed by using manual classification method．The reliability ofexperimental result WaS analyzed． I