小麦基部茎秆禾谷丝核菌DNA含量的定量检测.pdfVIP

江苏农业学报(JiangsuJ.of Agr.Sci. ),2015,31(4):750 ~755 750 http:/ / 周淼平,姚金保,张 鹏,等. 小麦基部茎秆禾谷丝核菌DNA含量的定量检测[J].江苏农业学报,2015,31(4):750-755. doi:10.3969/ j.issn.1000-4440.2015.04.007 小麦基部茎秆禾谷丝核菌DNA含量的定量检测 周淼平, 姚金保, 张 鹏, 杨学明, 余桂红, 马鸿翔 (江苏省农业科学院农业生物技术研究所/ 江苏省农业生物学重点实验室,江苏 南京 210014) 摘要: 为探索小麦基部茎秆中禾谷丝核菌DNA含量与其纹枯病抗性的关系,以掺入不同浓度梯度的禾谷丝 核菌DNA小麦基因组DNA为模板,采用荧光定量PCR方法建立了小麦基部茎秆禾谷丝核菌DNA定量测定方法。 通过该定量测定方法对42份室内接种禾谷丝核菌以及12份田间接种禾谷丝核菌小麦品种(系)的基部茎秆禾谷 丝核菌DNA含量进行了测定,并与其纹枯病平均病级进行相关性分析,结果表明,室内接种的幼苗基部茎秆禾谷 丝核菌DNA含量与其纹枯病平均病级数显著相关,相关系数为0.76。 田间接种的成株期基部茎秆禾谷丝核菌 DNA含量与其纹枯病平均病级相关性不显著。 该研究结果可为今后开展小麦幼苗的纹枯病抗性研究提供参考。 关键词: 小麦; 纹枯病; 荧光定量PCR方法 中图分类号: S512.1 文献标识码: A 文章编号: 1000-4440(2015)04-0750-06 Quantitative detection and application ofRhizoctonia cerealis DNA content in basal stalk of wheat ZHOU Miao-ping, YAOJin-bao, ZHANG Peng, YANG Xue-ming, YU Gui-hong, MA Hong-xiang (Institute of Agro-biotechnology,JiangsuAcademy of Agricultural Sciences / ProvincialKey Laboratory of Agrobiology,Nanjing 210014,China) Abstract: To explore therelationship betweenwheat sharp eyespotresistance andRhizoctoniacerealis DNA con- tent in basal stalk,wheat genomic DNA mixed with the gradient concentrations of Rhizoctonia cerealis DNA was used as template,and the quantitative determination method for R. cerealis DNA content in wheat background was devel- oped by real-time PCR. TheR. cerealis DNA contents in basal stalk of42 varieties inoculated indoor and 12 varieties inoculated in wheat field were then measured. The correlation between R. cerealis DNA content and the average dis- ease grade of sharp eyespot were also analyzed. The result showed that there was a significant correlation between R. cerealis DNA content and the average disease grade of sharp eyespot