基于碳纳米管的电化学生物传感分析-分析化学专业论文.docxVIP

基于碳纳米管的电化学生物传感研究 基于碳纳米管的电化学生物传感研究 V V cm -2 mM -1 ) ， low detection limit (3 μ M , S/ N=3) , wide li ner range ( 6 μ M ~ 1.2 mM) and good stab ility can also be obt ained at PG/CNTs/ C o O x -NP s electrode. This kind of m o di fie d elect rode ha s great pote ntial in nonenzym atic glucose detection. ( 2 ) The bor on-doped c a r bon na notube s ( BCNTs ) were used i n a m perome t r ic bios ens o r s . It has been found that the electrocatalytic activity of the BCNTs modi fied gl assy carbon el ect rode t o ward the oxidati on of hydr ogen peroxide is muc h higher t h an t h at of the un-doped CNTs modi fied electr o de due t o the la rg e a m ount of e dge sites and oxygen-ri ch gr oups locat ed at the defective sites i n duced by boron dopi ng. Glucose oxidase ( G OD) wa s se lected as the model enz y me a nd immobilized on the BCNTs modif i ed glassy car bon (GC) electrode by e n tr ap p i ng GOD in to P o ly ( o - a m i nophe nol) fil m . The per f or mance of the sensor was i n vesti g ated by electro chem i cal methods. At an opti m u m potential of +0.60 V a nd pH 7.0, the bios ensor exhibits good chara c teri stics, suc h as hi gh sensiti vit y ( 1 71.2 nA mM -1 ) , l o w det e ction lim it (3.6 μ M), short r e s ponse ti me ( w it hin 6 s) , satis fa c t ory anti-interf erence ability and good stability. The apparent Micha e lis -Mente n c onst a nt ( K m app ) is 15.19 mM. The applicability to the whole blood analysis of the enzym e electrode was also evaluated. (3) GC/ B CNTs ele c trode ha s bee n c onst r ucted. The catal ytic properti e s of the GC/ BCNTs electrode t o war d s oxi dation of L- cyst e i ne has been investigat ed by C V. GC/ BCNTs el ect rode i s compar abl y stable, and coul d be kept fr om fouling by se l ecti n g suitabl e potential. Compar ed with GC/CNTs electrode, the GC/B CNTs elect rode has shown