硫熏药材检控方法评述和创新研究策略探讨.docxVIP

硫熏药材检控方法评述和创新研究策略探讨孔铭徐亚运李松林南京中医药大学附属中西医结合医院中药质量研究室江苏省中医药研究院和中国中医科学院江苏分院代谢组研究室摘要：硫熏是药材杀虫防霉养护方法之一，但过度硫熏会影响药材品质，从而影响药材药效和安全。对硫熏药材进行检控是保证药材安全、有效的前提。本文对当前硫熏药材检控方法进行评述，特别对S02残留量等检控方法存在的不足进行了较深入分析，对硫熏药材特征标志物检控方法研究以及硫熏药材检控指标选择策略进行探讨，为深入研究硫熏药材检控方法和修订检控标准提供新思路。关键词：硫熏;中药材;二氧化硫残留;特征标志物;检控方法;作者简介：李松林Tel: (025)E-mail:songlinli64@126.com作者简介：孔铭Tel: (025)E-mail:kongmingl027@163. com收稿日期：2017年1月20日基金：国家自然科学基金资助项目(编Inspecting sulfur-fumigatedherbs:an overviewKONG Ming XU Ya-yun LI Song-linDepartment ofPharmaceutical Analysis,Aff订iated Hospital of Integrated Traditional Chinese and Wes tern Medicine, Nanjing Universi ty of Chinese Medicine;Abstract：Sulfur fumigation has been used to kill insect and emti-mildew for conserving Chinese medicinal herbs. However, excessive su 1 fur-fumigation affected the quality of the herbs, consequently their efficacy and safety. So inspecting sulfur-fumigated herbs is crucial to ensure the safety and efficacy of the medicineil herbs. Tn this paper, the current methods for inspccting sulfur-fumigated herbs were reviewed;in particular, the shortage of the sulfur dioxide residue test methods was critically commented. Furthermore, the possibility of using sulfur-fumigation induced characteristic chemical markers as reference compounds as wel1 as the strategy of seiecting the markers for specifically inspccting sulfur-fumigated herbs were extensivcly discussed, so as to provide new ideas for establishing or revising the standards for controlling the quality of sulfur-fumigated herbs.Keyword：sulfur fumigation; Chinese medicinal herbs; S02 residue; cherracteristic chemical marker; inspccting method;Received： 2017 年1 月20 日中药材硫熏有近百年历史［1-2］,曾合法应用于山药等少数药材加工养护宜。由于硫熏可杀虫防霉，漂白美化药材，加之操作简单，成木低廉，近年来呈现过度熏蒸和品种蔓延之势。研究证实中药材过度硫熏导致SO?、重金属等有害物严重超标［4-8］。研究还发现硫熏会引起药材中化学组分的改变［9-13］,进而影响其体内过程［14-15］和药理活性［16-17］,存在一定的安全隐患［18-19］。中国药典自2005年版起全面取消了中药材的硫熏工艺，并自2010年版起采用S02限量检控硫熏药材但我们在应用过程中发现，该方法存在一定局限性［21-22］。因此，建立更加科学、可行的硫熏药材检控方法显得尤为迫切。本文分析了当前各种