桂花花期及采后储藏过程中连翘脂素含量变化规律研究-生物化学与分子生物学专业论文.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文 I I 摘 要 连翘脂素是桂花浸膏中高含量的活性天然产物，具有抗氧化、降血脂等多种药 用活性，可以生产出具有较高经济价值的原料药，但相应的生产工艺研究较少。因 此，该文选题具有良好的实用价值。 继课题组前人研究桂花精油的基础上，以提取精油后的花渣为原料，首先是提 取和分离纯化，获得了纯度较高的连翘脂素，然后跟踪了桂花花期及采后连翘脂素 的含量变化规律。主要工作及结果包括： 1. 建立了连翘脂素的检测方法；优化了超声波辅助提取桂花中连翘脂素的条件， 确 定最佳的提取参数的组合；获得了纯度 98%的连翘脂素产品。 2. 对于连翘脂素的化学稳定性进行研究，确定了加工和储存过程中 PH、氧化还原 环境等对其含量影响的范围。结果显示，碱性环境、氧化剂、还原剂、酸味剂、 防腐剂都会对连翘脂素的结构造成影响。 3. 确定了桂花储藏过程中浸膏中连翘脂素含量主要受控于微生物的作用，因此在储 藏过程中应尽量抑制微生物的生长。 4. 在此基于上提出桂花储藏过程中保持连翘脂素含量的工艺措施是：在桂花盛花期 采摘、以 20%盐腌制联合 2%的柠檬酸或者 20%盐腌制联合使用 0.1mg/g Nisin 作 为保护剂。这一结果对桂花储藏技术的应用及研究具有一定的指导意义。 关键词； 桂花 连翘脂素 合成积累 提取纯化 稳定性 II II Abstract Phillygenin is an important pharmaceutical raw materials of O. fragrans. It has pharmacological activity, such as antioxidant, lowering blood pressure and others, which has high economic value. But the current research is less about technique of production of Phillygenin. Therefore the text has a very important significance. Our group has studied essential oils of O. fragrans. On this basis, we analyzed the chemical composition of the dregs. Firstly, We obtained Phillygenin from dregs of O. fragrans, and the purity is high. Next, tracking the content of phillygenin of O.fragrans during flowering and storage process, exploring the factors of content changes of phillygenin during storage of O. fragrans, and the following were the results of the research. The detection method of phillygenin of O. fragrans was established using HPLC. Optimize the technology of extraction and purification of phillygenin of O. fragrans, determine the optimum extraction conditions. We obtained phillygenin and the purity is 98%. The chemical stability of phillygenin was studied, The results showed that alkaline environment, oxidizing agents, reducing agents, sour agents and preservatives will damage structure of phillygenin. The reason for the yield change of phillygenin of O. fragrans during storage was researched. The result showed that enzyme and microbial had some impact on the yield of phillyg