海洋青鳉鱼肝脏对六价铬的解毒与抗氧化防御研究-遗传学专业论文.docxVIP

摘要Abstract 摘要 Abstract During recent years，with acceleration of the industrialization and the development of coastal Cities，wastewater from industry，agriculture and municipal ity pour into the coastal seawater，posing direct and indirect threats to marine organisms and human health．Marine medaka (Oryzia melastigma)is new promising experimental organism for marine ecotoxicology for being sensitive to the change of water and environment quality．In this study，we use the adult medaka’S 1iver and juvenile medaka as models to explore the mechanism of marine fishes response to chromium induced oxidative stress and detoxification．The main results follows． 1)Three—months adult marine medakas were exposed to 2．61mg／L， 5．22mg／L，lO．44mg／L，20．88mg／L，41．77mg／L that five hexavalent chromium concentration setting refers to the half lethal concentration of 50％ (LC50)，with exposure duration for 96 hours in seawater．ICP—MS analysis shows that in the brain，muscle and skeleton，there is a consistent concentration of chromium， and the bio—enrichment coefficient of liver greater that eight，while in 1iver and gut，chromium concentration increased with rising chromium concentration． 2)Three-months adult marine medakas were exposed to hexavalent chromium at the concentration of 2．61mg／L，5．22mg／L， 10．44mg／L，with exposure duration for 96 hours in seawater．We found the detoxifying system were activated in 1iver cells by testing glutathione S-transferase(GGT)and NADPH P450 reductase enzymes activity．The GST activity increased with the rising hexavalent chromium concentration．The total antioxidant capacity increased after exposure indicated the antioxidant defense system was activated 3)Three—months adult marine medakas were exposed to hexavalent chromium at the coneentration of 2．61mg／L，with exposure duration for 96 Ⅱ 万方数据 摘要hours．For 摘要 hours．For 1iver，the real—time PCR results showed the key transcription factor Nrf2 of Keapl—Nrf2 system and the antioxidative enzyme gene Cu／zn—sod,c