满天星组织培养中克服玻璃化现象的初探-广西植物.PDF

246 uihaia　17 (3):246—248　1997 ＊ 张燕玲　姚　军　王润珍　唐高凤 ( , 541006) 　　、 、 (BA KT)、 (PVA), , , , 2.0 g/L。 　;; A PRELIMINARY STUDY ON THE MEASURES TO OVERCOME THE VITRIFICATION OF GYPSOPHILA IN VITRO Zhang Yanling　Yao Jun　Wang Runzhen　Tang aofeng (Guang x i I nsti tu te of B otany , Guangx i Zh uangz u A utonomous R eg ion a nd A cademia S inica, uilin 541006) Abstract　From the experiments of mixing sugar, agar, BA and KT, PVA with different concentra- tions in the culture medium, we find out that the number of vitrified plants of Gyp sop h ila decreaseswith the increase of the concentrationsof sugar and agar, but BA and KT have not any effect on the vitrifica- tion of the plants in vitro of Gypsop hila ;and PVA has a very obvious inhibition on the vitrification of the plants in vitro of Gyp sop hila .Among of the treatments, the most suitable concentration is 2.0 g/ L PVA. Key words　Gypsop hila;tissue culture;vitrification (Gy psop h ila sp.), , 。, , 。, , 。 , 〔1〕 , 。 , 。 , , 。, , 〔2, 3, 4〕。 　　1995-08-09 　　:, , 1963 , , 。 　＊。 　3 :　 247 、 、 PVA 。 1　 1　材料与方法 Table 1　The relationship between various concentrations of sugar and the formation , 75% 0.5 of vitric plants 　　 min, 0.1%HgCl 6 min, 2 (g/L) () % 4 ～5 ;, 20 20 20 100 1cm ()MS+ 40 20 17 85 BA 0.5 m