玫瑰精油抗血栓形成作用-美国细胞修复系统医学中心.PDF

2 70 2011, Vol. 32, No. 11 食品科学 ※营养卫生 玫瑰精油抗血栓形成作用 厍文波，甘 露，安晓晶，马建慧，陈 虹，郑秋生，王振华* (新疆特种植物药资源教育部重点实验室，石河子大学药学院，新疆石河子 832002) 摘 要：目的：研究玫瑰精油对大鼠体外全血血小板聚集以及大鼠动- 静脉旁路血栓形成的影响。方法：大鼠 颈总动脉取血后，随机分为溶剂对照组，阿司匹林阳性对照组，玫瑰精油高( 10 0 μg/mL) 、中(50 μg/mL) 、低 (25 μg/mL)剂量组，分别采用血小板聚集诱导剂二磷酸腺苷(ADP) 、钙离子载体(A2 3 187)和CaCl2 激发血小板聚集， 以电阻法分别检测玫瑰精油对体外全血血小板聚集的抑制作用。取Wistar 大鼠50 只，均于手术前3d 进行分组灌胃 给药，即溶剂对照组，阿司匹林阳性对照组(30mg/ (kg ·d)) ，玫瑰精油(溶于注射用大豆油) 高( 100mg/ (kg ·d)) 、中 (50mg/(kg ·d)) 、低(2 5mg/(kg ·d))剂量组，然后采用大鼠动- 静脉旁路血栓形成法观察玫瑰精油对血栓形成的抑制 作用。结果：玫瑰精油能显著抑制AD P 、A2 3 187 、C aC l2 诱导的大鼠血小板聚集和动- 静脉旁路血栓的形成。 关键词：玫瑰精油；血小板聚集；血栓形成 Anti-thrombosis Effect of Rose Essential Oil SHE Wen -bo ，GAN Lu ，AN X iao-j ing ，MA Jian -hui ，CHEN Hong ，ZHEN G Qiu -sh eng ，WAN G Zh en-hua* (Key Laboratory of Xinjiang Endemic Phytomedicine Resources, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832002, China) Abstract : Objective: To investigate the effect of rose essential oil on whole blood platelet aggregation and arteriovenous shunt thrombosis in rats. Methods: The platelet aggregation in whole blood samples from rats was induced by adenosine diphosphate (ADP), calcium ionophore (A23 187) and CaCl2 , respectively. The inhibitory effect of rose essential oil at the doses of 25, 50 mg/L and 100 mg/L on platelet aggregation was measured by electric resistance method in vitro. Fifty male Wistar rats were randomly divided into 5 groups including the control group, aspirin positive control group and three groups respectively administered with rose essential oil at the doses of 25, 50 mg/kg and 100 mg/kg. After oral administration