毛细管电泳法测定S-羟基-2二氢苯并呋喃-基乙酸甲酯中R.PDF

·776 · 现代药物与临床 Drugs Clinic 第 32 卷 第 5 期 2017 年 5 月 毛细管电泳法测定(S)-2-(6-羟基-2,3-二氢苯并呋喃-3-基) 乙酸甲酯中R 异构体 潘文燕，闫玉刚，侯 准，杨新颖，方 浩* 山东大学 药学院，山东 济南 250012 摘 要：目的 建立毛细管电泳法测定(S)-2-(6-羟基-2,3-二氢苯并呋喃-3-基) 乙酸甲酯中R 异构体的方法。方法 采用毛细管 电泳法。以磺酸-β-环糊精（S-β-CD ）为选择剂；25 mmol/L 硼酸盐缓冲液（pH 8.9 ，含 S-β-CD 1.8% ）为运行缓冲液；运行 电压为25 kV ；柱温为 15 ℃；检测波长：214 nm ；3.4 kPa 压力进样 10 s。结果 (S)-2-(6-羟基-2,3-二氢苯并呋喃-3-基) 乙酸 甲酯与R 异构体的分离度为 2.9 。R 异构体在 2～30 μg/mL 与峰面积线性关系良好（r ＝0.999 5 ），平均回收率为 96.6%，RSD 值为 4.9% （n ＝6 ）。结论 建立的毛细管电泳法操作简便，结果准确可靠，可用于(S)-2-(6-羟基-2,3-二氢苯并呋喃-3-基) 乙酸 甲酯中R 异构体的控制。 关键词：(S)-2-(6-羟基-2,3-二氢苯并呋喃-3-基) 乙酸甲酯；R 异构体；毛细管电泳 中图分类号：R927.2 文献标志码：A 文章编号：1674 - 5515(2017)05 - 0776 - 04 DOI: 10.7501/j.issn.1674-5515.2017.05.005 Determination of R isomer in (S)-methyl-2-(6-hydroxy-2,3-dihydrobenzofuran-3-yl) acetate by capillary electrophoresis PAN Wen-yan, YAN Yu-gang, HOU Zhun, YANG Xin-ying, FANG Hao School of Pharmacy, Shandong University, Jinan 250012, China Abstract: Objective To establish a capillary electrophoresis method for the determination of R-optical isomer in (S)-methyl- 2-(6-hydroxy-2,3-dihydrobenzofuran-3-yl)acetate. Methods Capillary electrophoresis method was adopted. Sulfate-β-cyclodextrin (S-β-CD) was used as selector. The background buffer was 25 mmol/L borate buffer solution (pH 8.9, containing 1.8% S-β-CD). The separation was performed at 15 ℃ with the voltage of 25 kV and the wavelength was 214 nm. Pressure was 3.4 kPa and injected for 10 s. Results (S)-methyl-2-(6-hydroxy-2,3-dihydrobenzofuran-3-yl)acetate and its R-enantiomer were basely resolved with resolution of 2.9. The calibration curve of R-enantiomer was linear in the concentration range of 2 — 30 μg/mL (r = 0.999 5). The average recovery was 96.6% with RSD 4.9% (n = 6). Conclusion The method is convenient, rapid, accurate, and can be used for the determination of (R)-methyl-2-(