标准菌株CMCCF98003的多相复核鉴定-中国工业微生物菌种保藏.PDF

微生物学通报 NOV 20, 2010, 37(11): 1600−1605 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 标准菌株 CMCC(F)98003 的多相复核鉴定 姚粟 李辉 程池* ( 100027) 摘 要: 采用形态学观察、Biolog 全自动微生物鉴定系统、ITS rDNA 序列分析、ITS 区特殊位点 碱基分析、β-微管蛋白基因序列分析方法, 对《中国药典》(CHP) 中指定使用的培养基质控参考菌 株 CMCC(F)98003 进行了多相复核鉴定。结果表明: CMCC(F)98003 为黑曲霉, 与CHP 收载的科 学名称是一致的。鉴于黑曲霉与巴西曲霉虽然较为接近, 但已成为两个不同的种, 其形态学、系 统发育学和代谢产物等特性均存在一定差异, 《中国药典》是否需要更换新的丝状真菌代表菌株, 以保持与国际上其他药典的一致性值得研究。 关键词: CMCC(F)98003, 黑曲霉, 巴西曲霉, 多相鉴定 Polyphasic Identification Approaches for Reference Strain CMCC(F)98003 * YAO Su LI Hui CHENG Chi (China National Research Institute of Food Fermentation Industries, China Center of Industrial Culture Collection, Beijing 100027, China) Abstract: To identify reference strain CMCC(F)98003, which cited in Chinese Pharmacopoeia (CHP), polyphasic approaches including morphological features, Biolog identification system, ITS rDNA se- quence, specific positions based on the ITS region and β-tubulin gene sequences analysis were used. Results indicated that CMCC(F)98003 was identified as Aspergillus niger and consistent with the scientific name in CHP. Although A. brasiliensis is close to A. niger , they have been classified as two different species and their morphology, phylogenesis and metabolic profilies are distinct. To maintain the consistency of international Pharmacopoeia, whether CHP replaces the new representative of fila- m