海水绿藻和淡水绿藻光照产氢特征的比较研究-生物化工专业论文.docxVIP

AbstractAbstract Abstract Abstract Chunqiu RAN(Biochemical engineering) Directed by Prof．Wei ZHANG In this paper,systemically researched the characterization and mechanism of marine green algae Platymonas subcordiformis hydrogen photoproduction under stress Condition such as addition PSII inhibitor DCMU，sulfur-deprived，and treated witIl uncoupler CCCP,freshwater green algae Chlamydomonas reinhardtii ccl24 was taken as contrast sample，to obtain the suitable method of regulation hydrogen photoproduetion． DC～rU call markedly inhibit the PSII photochemical activity,and hold the suspended culture anaerobic condition,but it couldnt promote green algae hydrogen photoproduction．Comparing to the PSII photochemical activity of C reinhardt打was obviously inhibit by sulfur-deprived in medium．which of P subcordiformis only was slightly depressed,couldn’t photoproduce laydrogen． CCCP call inhibit the PSII photochemical activity of置subcordiformis,wbJeh close related to the concentration of cccP,the rate of hydrogen photoproduction obviously improved．Besides，CCCP Call promote C reinhardtii cel24 hydrogen photoproduetion too．but which clearly inferior to that of C reinhardtii cel24 by sulfur-deprived．There are dual effects on the PSII photochemical activity of C reinhardtii ecl24 by addition CCCP and sulfur-deprived in medium，but has low capacity ofhydrogen production． The effects of metabolism and electron source on hydrogen photoproduction of P subcordiform捃treated witIl CCCP after anaerobic dark incubation were investigated，which indicated that the metabolism of P subcordiformis was fermentation during anaerobic dark incubation and hydrogen photoproduction after addition CCCP，and about 90％electron need for hydrogen photoproduction Abstract Abstract come from water oxidation by PSII，the remaining 100,4 probably transfer from endogenous substrate degradation． Further study the effea of medium on endogenous substrate accumulation and hydrogen photoproduction by P subcordiformis．The