铈Ⅳ-抗坏血酸-奎宁化学发光分析体系测定抗坏血酸.PDF

※分析检测 食品科学 2009, Vol. 30, No. 24 409 唐志华，熊海涛 ( 陕西理工学院化学与环境科学学院，陕西汉中 723001) ：基于在酸性条件下Ce(Ⅳ) 能氧化抗坏血酸产生微弱化学发光，加入奎宁后能显著增强其发光强度，并且 发光强度与抗坏血酸浓度在一定范围内呈线性关系，建立流动注射化学发光分析法测定抗坏血酸。在最佳实验条件 下，该法线性范围为0.03～1.0mg/ml，检出限为0.012mg/ml ，对0.3mg/ml 的抗坏血酸溶液平行测定11 次，其相对 标准偏差(RSD)为2.5% ，可成功应用于VC 针剂的测定。 ：Ce( Ⅳ)- 抗坏血酸- 奎宁；化学发光；流动注射 Determination of Ascorbic Acid by Ce(IV)-Ascorbic Acid-Quinine Chemiluminescence Analytical System TANG Zhi-hua ，XIONG Hai-tao (School of Chemistry and Environmental Science, Shaanxi University of Technology, Hanzhong 723001, China) Abstract ：In acidic condition, oxidation of ascorbic acid by Ce (IV) resulted in a poor chemiluminescence, and the addition of quinine exhibited a significant enhancement of luminescence. Furthermore, an excellent linear range between luminescence intensity and ascorbic acid concentration was observed. Thus, a flow injection chemiluminescence analytical method for determining ascorbic acid was developed. Under the optimal condition, the liner range for ascorbic acid was 0.03－1.0 mg/ml with a detection limit of 0.012 mg/ml. The relative standard deviation was 2.5% for 0.3 mg/ml ascorbic acid solution among 11 parallel determinations. Therefore, this proposed method can successfully used to determine quinine injection and tablets. Key words ：Ce (Ⅳ)-ascorbic acid-quinine ；chemiluminescence ；flow injection 中图分类号：O657.3 文献标识码：A 文章编号：1002-6630(2009)24-0409-03 VC 也称为抗坏血酸，在人体内参与多种反应，尤 化学发光分析体系种类较多，以强氧化剂作为化学 其在生物氧化和还原以及细胞呼吸中起着重要作用。当 发光试剂建立起来的化学发光分析法还具有反应体系简 VC 缺乏所引起的坏血病时，伴有胶原合成缺陷，表现 单、试剂便宜易得、用于分析的对象较广的优点。与 为创伤通常难以愈合，牙齿形成障碍和毛细血管破损引 KMnO4 化学发光反应相比，利用Ce(I