桂枝抗过敏活性成分的研究-制糖工程专业论文.docxVIP

桂枝抗过敏活性成分的研究摘要 桂枝抗过敏活性成分的研究 摘要 本文以传统中药桂枝为研究对象，以透明质酸酶抑制率来评价其抗过 敏作用，通过溶剂提取、萃取分离、柱分离纯化的方法获得透明质酸酶抑 制率较高的样品，通过薄层鉴定、颜色反应、紫外、红外光谱及LC．MS联 用技术对纯度较高的样品进行初步的结构鉴定。同时，跟踪桂枝提取物对 羟基自由基、过氧化氢、超氧阴离子和DPPH·的清除率，通过与透明质酸 酶抑制率的对比研究，初步预测了桂枝抗过敏作用的机制，并对抗过敏植 物的筛选提供了一定的依据。实验结果如下： 桂枝抗过敏成分的提取条件为桂枝粉碎过20目筛，70％乙醇溶液提取， 提取温度为70℃，料液比为1：10，1．0h／次，重复提取3次；提取液依次通过 氯仿、乙酸乙酯、正丁醇萃取，获得抗过敏活性较强的组分，将此组分通 过大孔树脂、聚酰胺和葡聚糖凝胶进行分离纯化，得到抗过敏活性最强的 组分桂15，其酶抑制率高达93％(1．2mg／mL)，总酚含量为90％。 桂15在薄层层析板上展开后喷上三氯化铁．铁氰化钾溶液显示为蓝色， 颜色反应证明其为缩合单宁类物质，紫外、红外光谱也显示多酚特性，通 过液质联用技术进一步确定桂15中含缩合单宁的代表酚儿茶素和表儿茶 素。 本实验同时跟踪了样品的抗氧化性，厂结论是抗过敏性强的样品具有较 强的抗氧化性，这对抗过敏植物的筛选和抗过敏作用的机制的研究具有一 定的启发作用。 关键词：桂枝抗过敏提取纯化结构鉴定儿茶素表儿茶素抗氧化 THE THE RESEARCH oF ANTIALLERGIC INGREDIENT OF RAMULUS CINNAMoMI ABSTRACT In this paper，the Ramulus Cinnamomi belonging to Chinese traditional medicine was studied．The inhibition ratio of hyaluronidase was used to evaluate the antiallergic effects of samples．Solvent extract，extraction and Column Purification were used in order to get the sample that had high antiallergic effect． Also thin layer chromatography，color reaction，UV，IR and LC—MS were used to identify the structure of sample that had high antiallergic effect．At the same time， the scavenging ratio of Hydroxyl free radical，hydrogen peroxide，superoxide anion free radical and DPPH·of samples was followed，via the contract between the inhibition of hyaluronidase and the scavenging ratio，the mechanism of antiallergic effects of Ramulus Cinnamomi was forecast，also some bases were provided for US to filtrate the antiallergic plant． The best extract conditions were chosen as follows：The Ramulus Cinnamomi was breaked into pieces，then filtered by 20 meshes bolt，the extract temperature was 70。C，sample weight to solvent volume was 1／1 0，every extraction was for 1 hour and the extraction were duplicated three times，Then the extract was leached by chloroform，acetic ether，n—butyl alcohol，the sample which had higher antiallergic effect was isolated by HP-20，polyamide and sephadex LH-20，then the sample o