寒兰试管成花的形态解剖学研究-植物学专业论文.docxVIP

缩略词缩略词 缩略词 缩略词 UST OF ABBREⅥA耵ON abscisic acid 脱落酸 acenosine triphosphate 腺苷三磷酸 6-benzyladenine 6．苄基腺嘌呤 formalin．acetic acid—alcohol 福尔马林．冰醋酸．酒精 gibberellin 赤霉素 uniconazole 优康唑 thidiazuron 苯基噻二唑基脲 a—naphthaleneacetic acid a．萘乙酸 撇册～眦姒一僦～M tool 摩尔 L Liter 升 k lux 勒克司 second 秒 minute 分 s疵h hour 小时 d day 天 rotation 转 milligram 毫克 gram 克 millimeter 毫米 f哗g～肛 microliter 微升 可4 可 ?-aavd ? ? ? 摘要摘要 摘要 摘要 ．～- 本文以寒兰试管苗为材料，采用石蜡切片和透射电镜的方法，观察了寒兰试 管成花过程中的形态发生和结构发育过程；以寒兰试管花为材料，对花的育性进 行了初步研究。结果如下： 寒兰试管花的发育可划分为花芽分化前期、苞片分化期、花原基分化期、花 被分化期、合蕊柱分化期、花粉囊和柱头形成期6个阶段；寒兰试管花萼片和花 瓣结构相似，唇瓣结构特殊；合蕊柱中问为扁平的花柱道；花药由药隔和2对花 粉囊组成；子房1室，有3个侧膜胎座；寒兰试管成花过程中，淀粉分布越向花 芽生长中心越少，蛋白质则集中分布在花芽生长中心；寒兰试管成花过程中，生 长锥细胞的细胞核、核仁、线粒体、内质网、液泡等细胞器的大小，数量等均发 生了明显的变化；寒兰试管花花粉离体萌发的最佳培养基为100 mg L-1硼酸+300 mg r1硝酸钙+100 mg U1硝酸钾+200 mg L-1硫酸镁+1％蔗糖+0．5％琼脂，最高 萌发率为46．11％．寒兰试管花的柱头均具有可授性，寒兰试管花在完全盛开时 是人工授粉的最佳时期。 关键词：寒兰；试管成花；显微结构：超微结构；花粉萌发． Abstract Abstract ■ In this paper,the test-tube seedlings of Cymbidium kanran Makino／n，拓加werc 0^ used as experimental material to study the differentiation and development processes of flower bud by paraffin method and scanning electron microscope，and the f10wer 0f Cymbidium kanran Maldno／n vitro were used as experimental material to studv the flower of fertility．The main results were as followings： The process of flower bud differentiation and development of CymbidiH，挖ka以，口n Makino／n vitro flowering has been divided into 6 stages by correlating morphological traits of the flower buds：prophase of flower bud differentiation，di骶rentiation of floral primordial，differentiation of perianth，differentiation of coIu眦，fbnnation of pollinia and stigma．Microstructure of sepal and petal of Cymbidium ka．悖阳甩Mal(ino in vitro flowering is similar，aS the microstructure of labellum is special：Thin衄d nat stylar cannal is in the middle of column；Anther is composed of two pairs of pollinia and connective；Ovary is parietal placentation；During the process of Cvmbidi瞻m kanran Makino／n vitro flowering，protein content gradually incre