高效液相色谱法测定附子中尿嘧啶含量的方法研究-吉林农业大学学报.PDF

DOI :10.13327/j.j jlau.2003.03.017 吉林农业大学学报　2003, 25 (3):301～302, 306 Journal of Jilin Agricultural University 1 2 1 1 1 1 赵宏峰 , 洪　波 , 司云珊 , 解生旭 , 韩　冬 , 徐东铭 (1.吉林省中医中药研究院, 吉林长春130021;2.吉林农业大学资源与环境学院, 吉林 长春 130118) 　:采用反相高效液相色谱法, 次建立了附子中尿嘧啶含量的测定方法。 该方法线性关系良好(r= 0.999 8), 平均回收率为100.04%, RSD=1.84%。 该方法简单、准确、重现性好, 适用于附子中尿嘧啶的定量 分析。 :附子;尿嘧啶;高效液相色谱法 :R284.1　　　:A　　　:1000-5684(2003)03-0301-02 Studies on Quantitative Determination of Uracil Content in Ac onitum carmichaeli by RP-HPLC Content 1 2 1 1 1 1 ZHAO Hong-feng , HONG Bo ,SI Yun-shan , XIE Sheng-u , HAN Dong , XU Dong-ming (1.Jilin Academy of Traditional Chinese Medicine and Material, Changchun, Jilin 130021, China ; 2.College of Resource and Environmental Science, J ilin Agricultural University, Changchun, J ilin 130118, China ) Abstract:A new method for the quantitative determination of uracil content in Aconitum carmichaeli with RP-HPLC was set up for the first time.The linearity of this method was quite satisfactory (r = 0.999 8), and the average recovery rate was 100.04%, RSD =1.84%.The method was simple, accu- rate, reproducible, and appropriate for the quantitative determination of uracil content in Aconitum carmichaeli Key words:Aconitum carmichaeli;uracil;RP-HPLC 　　(Aconitum carmichaeli Deb .) 。 , , 1　 。, [1-2] 。, Waters -600 (), Waters-486 (), PC-800 [3-4] 。 , AG-245 (), , KQ-250B ()。 , 、、、 :; , :;(