发光细菌法检测大米中的重金属.pdfVIP

2016 年第 29 卷第 5 期 粮食与油脂 63 发光细菌法检测大米中的重金属 何　早，吴卫国，胡雨欣，刘　霞 （湖南农业大学食品科技学院，食品科学与生物技术湖南省重点实验室，　湖南长沙　4 10128 ） 摘　要：以明亮发光杆菌（P.phosphoreum ）为受试物，对大米中的铅、镉、汞三种重金属进行了检 测。结果表明，发光抑制率与重金属溶液的质量浓度呈正相关，相关系数大于0.96 ，各重金属离子 对P.phosphoreum 的毒性大小排序为Hg2+ ＞Pb2+ ＞Cd2+ 。菌液与各重金属标准溶液加样体积比 为1 ∶1，检测时间为30 min ；HgCl 的线性范围为0.02~1.6 mg/L ，Pb （NO ）的线性范围为2~ 2 3 2 20 mg/L ，CdCl2 的线性范围为2~64 mg/L ；样品加标回收率范围为70.12%~93.5%，相对标准偏 差范围为5.31%~13.25%。该检测方法简便，成本低，精确度较高，测量结果直观。 关键词：明亮发光杆菌；重金属；大米 Study on the detection of heavy metals in rice using luminescence bacteria HE Zao ，WU Wei-guo ，HU Yu-xin ，LIU Xia （College of Food Science and Technology ，Hunan Province Key Laboratory of Food Science and Biotechnology ，Hunan Agricultural University ，Changsha 410128 ，Hunan ，China ） Abstract ：The lead ，cadmium and mercury were detected using luminescent bacteria test. The results showed that the light inhibition rate was positively correlated with the concentration of heavy metal solution ，and the correlation coefficient was higher than 0.96. The list of the toxicity sequence was Hg2+ ＞ 2+ 2+ Pb ＞Cd .Bacteria and heavy metals standard solution was loaded volume ratio of 1 ∶1，detection time was 30 min. The linear working range was 0.02~1.6 ，2~20 ，2~64 mg/L for HgCl ，Pb （NO ） 2 3 2 and CdCl . Spiked tests revealed that recoveries were in the range of 70.12%~93.5% with the relative 2 standard deviation of 5.31%~13.25%. This method was simple ，low cost and high accuracy. Key words ：P.phosphoreum ；heavy metal ；rice 1008―9578（2016）05―0063―04 中图分