人参皂苷Rg1参与诱导小鼠多潜能干细胞的研究.PDFVIP

人参皂苷Rg1 参与诱导小鼠多潜能干细胞的研究 1 2 1 1 #1 胡文涛 闫秋月 方瑜 邱占东 张苏明 摘要 目的：观察人参皂苷Rg1对鼠胚成纤维细胞MEFs作用后对其向 小鼠诱导多潜能干细胞 （iPS ）诱导转化效率的影响。方法：实验分 为5组Rg1 （0μg/ml 、0.3μg/ml 、1μg/ml 、3μg/ml 、10μg/ml ），5组均 予经典四因子的逆转录病毒载体感染，人参皂苷Rg1在细胞感染后前 3天内在MEFs培养基里加入，进行iPS细胞诱导，并对其进行鉴定。 结果：人参皂苷Rg1 （1μg/ml ）组可明显提高iPS 的诱导效率，诱导效 率为（0.045±0.00 19）%, 不添加组为 （0.01±0.0033 ）% ，所获iPS 细 胞经鉴定为阳性。结论：人参皂苷Rg1可能在提高小鼠iPS细胞的诱导 效率方面发挥了一定作用。 关键词 人参皂苷Rg1 ；诱导多潜能干细胞；细胞重编程；鼠胚成 纤维细胞 1 Roles of Ginsenoside Rg1 in the Induction of Mouse Induced Pluripotent Stem Cells HU Wen-tao ▲, YAN Qiu-yue, FANG Yu, QIU Zhan-dong, ZHANG 1作者单位：1.430030 湖北省武汉市华中科技大学同济医学院附属同济医院神 经内科 2.061000 河北省沧州市中心医院神经内科 基金项目 国家自然科学基金资助项目（No；中国博士后科学基金 资助项目（No.20110490453) #通讯作者 张苏明 邮箱 suming_zhang@126.com Su-ming. ▲Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China Abstract Objective: To observe the effects of Ginsenoside Rg1 on the induced efficiency of mouse induced pluripotent stem(iPS) cells. Methods: The experiments were divided into 5 groups Rg1 （0μg/ml 、 0.3μg/ml 、1μg/ml 、3μg/ml 、10μg/ml ）. The 5 groups were infected with retro-viruses expressing classic 4 factors while the medium of the experimental group was added Ginsenoside Rg1 during the first 3 days after infection. Then the clones were identified. Result: Ginsenoside Rg1 （1μg/ml ）can obviously improve the induction efficiency of iPSCs, The induced efficiency was (0.045±0.0019)% while induced efficiency of the control group was (0.01±0.0033)%. The clones were positive. Conclusion: We consider that Ginsenoside Rg1 might facilitate the inducing efficiency of iPS cells. Key words Ginsenoside Rg1; induced pluripotent stem cells; cellular reprogramming; Mouse embryonic fibroblasts cells. 2006年Takahashi和Yamanaka将成