基于棕榈油酸和异丁烯醛双指标鉴别地沟油的方法.PDF

※分析检测 食品科学 2013, Vol.34, No.08 199 基于棕榈油酸和异丁烯醛双指标 鉴别地沟油的方法 1 1 2 1 1,* 李 涛 ，朱小红 ，盛喜霞 ，刘海静 ，杨智海 (1.陕西省食品药品检验所，陕西 西安 7 10061；2.兵器工业卫生研究所，陕西 西安 7 10065) 摘  要：寻找地沟油中的特异性指标，根据其量值高低识别食用油和地沟油。实验采取两种路径：一是样品经皂 化、甲酯化后分析脂肪酸类成分；二是直接采用顶空进样富集样品中的挥发性成分，然后采用气相色谱-质谱法分 析各化学成分的量值规律。研究发现，棕榈油酸与异丁烯醛在地沟油中特异性较强。在所测定样本中，以棕榈油 酸单独鉴别时，总体检出正确率为54.1%，阳性样本检出正确率为100.0%、漏检率为60.7%，阴性样本检出正确率 为100.0%；以异丁烯醛单独鉴别时，总体检出正确率为78.4%，阳性样本检出正确率为100.0%、漏检率为28.6%， 阴性样本检出正确率100% 。二者协同鉴别，总体检出正确率为86.5%，阳性样本检出正确率为100.0%、漏检率为 17.9%，阴性样本检出正确率100.0%。棕榈油酸和异丁烯醛作为鉴别地沟油的特异性指标，两项检测结果能够相互 印证、综合判断，可较为准确地分辨地沟油和食用油。 关键词：地沟油；食用油；棕榈油酸；异丁烯醛；气相色谱-质谱法 Identification of Drainage Oil Based on Palmitoleic Acid and Methacrolein 1 1 2 1 1,* LI Tao ，ZHU Xiao-hong ，SHENG Xi-xia ，LIU Hai-j ing ，YANG Zhi-hai (1. Shaanxi Institute for Food And Drug Control, Xi ’an 7 10061, China ； 2. Institute of Industrial Hygiene of Ordnance Industry, Xi ’an 7 10065, China) Abstract ：This study was conducted to find out specific indicators for discriminating drainage oil from edible oil. For this purpose, tow approaches were proposed: one was analysis of fatty acid composition following sample saponification, and the other was methyl esterification and direct enrichment by headspace sampling method and GC-MS analysis of volatile components in samples. Results revealed that palmitoleic acid and methacrolein showed strong specificity in drainage oil. In the studied samples, using palmitoleic acid alone for identification, the overall correct detection ra