研究报告不同剂量野百合碱联合异丙肾上腺素对大鼠血流动力学变化.DOC

研究报告 不同剂量野百合碱联合异丙肾上腺素对大鼠血流动力学变化及心指数、右心肥厚指数研究 邹习俊，杨红宇，江滟，智妍，兰露莎，朱颜鑫，邹乾 （贵州医科大学实验动物中心 贵阳 550004） 【摘要】目的 研究不同剂量野百合碱联合异丙肾上腺素对大鼠血流动力学变化及心指数、右心肥厚指数。方法 采用SD大鼠64只，雌雄各半，体重200~250 g，随机分为空白对照组（n = 16）；模型组（n = 48）。模型组分为高剂量组（n = 16）: 80 mg/kg野百合碱采取腹腔一次性注射；10 mg/kg异丙肾上腺素采取腹腔连续注射1周；中剂量组（n = 16）：55 mg/kg野百合碱采取腹腔一次性注射；8 mg/kg异丙肾上腺素采取腹腔连续注射1周；低剂量组（n = 16）：30 mg/kg野百合碱采取腹腔一次性注射，3 mg/kg异丙肾上腺素采取腹腔连续注射1周。动物饲养到第6周后，行肺动脉压和右心室内压检测，心系数和右心室肥厚指数测定。结果 与对照组比较，低剂量平均肺动脉压和右室收缩压均无差异变化；中剂量组平均肺动脉压、右室收缩压均有差异变化（P0.05），低剂量组心系数和右心室肥厚指数均无差异显著。中剂量组心系数差异极显著（P0.01），右心室肥厚指数差异显著（P0.05）。结论 说明采用一次性注射野百合碱55 mg/kg，联合连续注射8 mg/kg异丙肾上腺素1周。既保证了大鼠的存活率，又保证了肺动脉高压的成功形成，野百合碱和异丙肾上腺素能对大鼠产生肺动脉高压，导致心气虚弱。 【关键词】野百合碱；异丙肾上腺素；大鼠；血流动力学；心指数；右心肥厚指数 【中图分类号】R-33【文献标识码】A【文章编号】1671-7856（2016）10-0000-00 Doi:10.3969/j.issn.1671-7856.2016.10.000 Effect of different doses of monocrotaline in combination with isopropylarterenol on the hemodynamic response, heart index and right heart hypertrophy index in rats ZOU Xi-jun,YANG Hong-yu, JIANG Yan, ZHI Yan, LAN Lu-sha, ZHU Yan-xin, ZOU Qian (?Laboratory?Animal?Center?of?Guizhou?Medical?University,? Guiyang 550004, China) 【Abstract】 Objective To study the effect of different doses of monocrotaline in combination with Isopropylarterenol on the hemodynamic resonse, heart index and right heart hypertrophy index in. rats . Methods Sixty-four healthy adult SD rats, male : female = 1:1, body weight 200-250 g, were randomly divided into blank control group (n = 16) and three model groups (n = 16 in each group). The high dose model group (n = 16) received i.p. injection of monocrotaline 80 mg/kg once and epinephrine 10 mg/kg once daily for one week. The moderate dose group received i.p. injection of monocrotaline 55 mg/kg once and epinephrine 8 mg/kg once daily for one week. The low dose group received i.p. injection of monocrotaline 30 mg/kg once and epinephrine 3 mg/kg once daily for one week. The rats were fed for 6 weeks, and then pulmonary artery pressure and right ventricular pressure were tested