少突胶质细胞体外培养方法的研究进展.PDF

中国康复理论与实践2017年5月第23卷第5期 ChinJRehabilTheoryPract,May, 2017,Vol.23,No.5 - 543 - DOI: 10.3969/j.issn. 1006-9771.2017.05.011 ·综述 · 少突胶质细胞体外培养方法的研究进展① 孙争宇，顾丽红，李林 [摘要] 寻求纯度高、简便易行的体外培养少突胶质细胞的方法具有非常重要的应用价值。本文综述大鼠皮层胶质细胞混合培 养、少突胶质前体细胞增殖纯化、少突胶质细胞诱导分化及细胞体系各发育分化阶段细胞表面抗原的鉴定等体外培养方法。 [关键词] 少突胶质细胞；少突胶质前体细胞；细胞培养；综述 Progress in Methodology for Oligodendrocyte Culture in Vitro (review) SUN Zheng-yu, GU Li-hong, LI Lin Department of Pharmacology, Xuanwu Hospital of Capital Medical University; Beijing Engineering Research Cen- ter for Nerve System Drugs; Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China Correspondence to LI Lin. E-mail: linlixw@ 126.com Abstract: It is important to establish a simple method of culture in vitro to obtain purity oligodendrocyte. This paper introduced the re- searches about co-culture in vitro with rat cortical gliocyte, proliferation and differentiation of oligodendrocyte progenitor, differentiation of oligodendrocyte and identification of cell surface antigens at different stages of development. Key words: oligodendrocyte; oligodendrocyte precursor cell; cell culture; review [中图分类号] R741 [文献标识码] A [文章编号] 1006-9771(2017)05-0543-05 [本文著录格式] 孙争宇，顾丽红，李林. 少突胶质细胞体外培养方法的研究进展[J]. 中国康复理论与实践, 2017, 23(5): 543-547. CITED AS: Sun ZY, Gu LH, Li L. Progress in methodology for oligodendrocyte culture in vitro (review) [J]. Zhongguo Kangfu Lilun Yu Shijian, 2017, 23(5): 543-547.