广西山羊无浆体初步研究及PCR诊断方法的建立-预防兽医学专业论文.docxVIP

广西人学硕士论文 广西人学硕士论文 广两山羊无浆体初步研究及PCR检测方法的建立 柳州、大化、都安的山羊临床血样共121份，同时应用本研究建立的PCR 诊断方法和姬姆萨染色后光学显微镜检验两种方法对样品进行检验，以比 较两种方法检测无浆体的准确性。结果用镜检的方法检测出阳。性20份，用 PCR的方法检测出阳性9份，低于镜检的阳性率，发现镜检时容易受条件 或主观判定标准影响而产生假阳性或假阴性从而导致判定的结果的准确性 降低。以PCR方法为标准，南宁和大化没有检验到阳性样品，柳州阳性率 为6．3％，都安阳性率为20．6％，这一结果表明了山羊无浆体的感染在广西 部分地区存在，而且有的地方感染情况较为严重，应进行深入广泛的调查 并采取有效的防治措施。 关键词：山羊绵羊无浆体16SrRNA基因系统进化分析PCR诊断方 法流行病学 广西大学硕士论文 广西大学硕士论文 广西山羊无浆体初步研究及PCR检测方法的建立 STUDIES FOR ANAPLASMOSlS OF GOArS IN GUANGXI AND THE DEVELOPMENT OF ITS PCR DETECTION TECHNIQUE In this study，one strain of Anaplasma was isolated from naturally infected goat in Du’all of Guangxi．The 16SrRNA gene sequence of this isolate Was analysized by the preparation of genome DNAs，PCR amplification，sequencing and compared to 23 strains of Anaplasma deposited in GenBank to inferred a phylogenetic tree．The A ovis isolated in Guangxi were separated into an A． ovis duster with other 6 strains from China，America and South Africa，the closest sequence was 16S rRNA gene of A．ovis—Idaho AF318945 from America， with a similarity of 100％．The analysis demonstrated that Anaplasmosis ovis was discovered in Guangxi first at molecular level，and this strain was same of A． ovis·Idaho AF318945 from America possibly． Based Oil the sequence of this research and the published sequence of 16S rRNA gene ofAnaplasma，a pair of primers of a PCR diagnostic method were designed and synthesized for epidemiological investigation of Anaplasmosis in Guangxi．PCR detection technique Was suit to detect earlier or invisible 广西人学硕士论文 广西人学硕士论文 厂西山羊无浆体初步研究及PCR检测方法的建立 infection of Anaplasmosis demonstrated by the specific test and sensitive test． And the blood sample could be detected store at 4C six monthes or ordinary temperature 2 monthes． In the epidemiolos investigation of Anaplasmosis ovis，121 blood samples collected from Nanning，Liuzhou，Du’an，Dahua of Guangxi were detected by PCR diagnostic method and the test under microscope．There are 9 to the by PCR diagnostic method and 20 to the test und