固定化热带假丝酵母载体的制备及其降解废水中苯酚的特性研究-生物化工专业论文.docxVIP

中文摘要阐述了固定化技术的原理、应用方法。研究了以海藻酸盐凝胶(ALG)和聚 中文摘要 阐述了固定化技术的原理、应用方法。研究了以海藻酸盐凝胶(ALG)和聚 乙烯醇(PVA)为主要包埋材料的混合载体固定化热带假丝酵母菌(Candida tropicalis)细胞处理工业废水中苯酚的关键性技术条件，解决了妨碍固定化细胞 在工业废水处理领域推广应用所面临的制备成球难，活性丧失大、易破碎，发胀 粘连上浮等问题。固定化细胞的最适pH值为6．0，最适培养温度为30℃。扫描 电镜观察到菌种在海藻酸钙包埋载体中能良好地生长和繁殖。 研究了热带假丝酵母降解苯酚的过程中底物苯酚随时间的变化，建立了苯酚 降解动力学模型；在摇瓶降解的基础上，对热带假丝酵母菌自由细胞进行了小型 发酵罐的苯酚及溶氧双底物动力学的研究，应用线性回归的方法得到了溶氧限制 常数鼠。 建立了一个数学模型来模拟ALG+PVA固定化细胞降解苯酚的过程，模型 考虑了苯酚和溶氧在包埋载体内、外部的传质阻力及苯酚一溶氧双底物本征动力 学。通过比较模型模拟与液相主体中实际测得苯酚浓度的实验数据验证其有效 性。此外，模型对ALG凝胶小球内苯酚、溶氧、细胞浓度随时间和半径的变化 关系做出合理的预测。 关键诃：热带假丝酵母菌；海藻酸凝胶；聚乙烯醇；固定化细胞；苯酚；废水处 理；动力学；扫描电子显微镜 ABSTRACTThe ABSTRACT The principle and application of immobilization are elucidated．The key conditious under which cell was immobilized with a mixed carried process using algirlate gel and polyvintl alcohol(PVA)as major entrapping material were studied． It was found that the mixed carrier process effectively solved problems that the immobilized cell techonology applied in wastewater treatment faced，such as difficulty in forming spherical particles，ease of the particles to be broken,loss of the activity in producing methane and floatation of the particles．The optimum PH of the immobilized ceils was 6．0 and the optimum temperature Was 30C．SEM images demonstrated that the bacteria immobilized in calcium mginate Can grow and reproduce well． The study Was conducted to evaluate the degradation kinetics of yeast．A degradation kinetiCS model Was found．Based on tlle flask culture conditions,the phenol degradation w盱e performed in a small fermentor．The kinetics of degradation were studied．The dissolved 02 kineticrate constant(go)weft determined by using a linear estimation method． A mathematical model Was proposed to simulate the batch phenol biodegradation process in immobilized system，which took into account the internal and external mass transfer resistances of phenol and oxygen and the double—substmte phenol-oxygen intrinsic kinetics．The validation of this model Was done by the comparison between the model