含山嵛酸低热量油脂的制备和评价-粮食、油脂及植物蛋白工程专业论文.docxVIP

AbstractAbstract Abstract Abstract Physicochemical properties and functional characteristic of Reduced-calorie fats containing behenic acid are similar to the natural fats and oils．Due to the poor absorption of behenic acid the caloric availability of fats iS reduced．Triacetate，methyl behenate and sunflower seed oil were used as the raw materials to prepare reduced—calorie fats in this research．Preparation technology,functional properties，caloric availability of reduced—calorie fat and its effect on serum lipid levels of rats were studied． A two—step transesterification process of preparing reduced—calorie fats containing behenic acid Was established，wherein triacetate was chemically transesterified with methyl behenate，then the resulting tribehenin Was enzymatically transesterified with sunflower seed oil to give reduced．calorie fats．The optimum parameters for chemical transesterification， purification of tribehenin，and enzymatic transesterification were respectively determined as follows：dosage of sodium methoxide 0．4％，reaction temperature 80 oC，reaction time 60 min， and substrate molar radio 3．2：l，Under the conditions the content of tribehenin Was up to 75％； re．crystallization temperature 30 oC，crystallization time 20 min，solid—liquid radio l1 2，and re—crystallization 3 times，Under the conditions the purity and recovery rate of tribehenin were both more than 90％：dosage of lipase 8％，reaction temperature 70 oC，and the reaction could reach equilibrium in 4 h． Physicochemical properties，such as solid fat content，crystallization properties， triglyceride composition，fatty acid composition，etc，were studied and analyzed．The results revealed that reduced．calorie fats containing a certain ratio behenic acid could be used as the base oils of margarine／shortening．For example，the plastic fat which was produced by 30％ tribehenin and 70％sunflower seed oil could be directly used as shortening．When the ratio of the tribehenin decreased to 20％．the plastic fat could be used to pr