因为肥胖不仅是由脂肪细胞数量增加-宜兰大学食品科学系-国立宜兰.DOCVIP

國立宜蘭大學食品科學研究所 碩士班專題討論 演講者：陳雅君 (Chen, Ya-Chin) 題目：白藜蘆醇和vitisin A抑制3T3-L1前脂肪細胞株之脂肪新生相關研究 指導教授：林世斌 老師 報告日期：Mar. 23, 2009 Study on the inhibition of adipogenesis of 3T3-L1 pre-adipocyte by resveratrol and vitisin A. 中文摘要 脂肪細胞分化一直被用來作為抗肥胖研究的標靶之ㄧ。肥胖不僅是脂肪細胞數量增加，脂肪細胞體積增大也是主要原因。過去20年，脂肪細胞分化的細胞及分子機制已被廣泛地研究。我們已知ㄧ個完整的脂肪新生過程，乃由前脂肪細胞增生繼而分化成脂肪細胞所構成。這表示透過抑制細胞增生及降低主要分化蛋白，如C/EBP、PPAR及SREBP，即可抑制脂肪新生。近年來，許多的報告顯示，類黃酮具有抑制3T3-L1新生脂肪細胞的能力，包括白黎蘆醇(resveratrol)及vitisin A。結果顯示，白藜蘆醇抑制3T3-L1脂肪新生與Sirt1的增加有關，Sirt1會抑制PPARγ促進脂肪代謝。然而，vitisin A會使p21-及Rb-dependent細胞週期停滯，進而抑制前脂肪細胞增生，達到抑制脂肪新生的效果。 關鍵字：類黃酮、白藜蘆醇、vitisin A、細胞分化、脂肪新生。 Abstract Adipocyte differentiation has often been a target of anti-obesity strategies, because obesity is caused not only by hypertrophy of adipocytes, but also by adipocyte hyperplasia. For the last 20 years, the cellular and molecular mechanisms of adipocyte differentiation have been extensively studied using preadipocyte culture systems. We have already known that the entire adipogenic process consists of the preadipocyte proliferation and their differentiation into mature adipocytes. Adipogenesis can be inhibited by reducing cell proliferation and decreasing major differentiation proteins, for example, C/EBP, PPAR and SREBP. Rrecently, many repoter have been shown that flavonoids inhibit adipogenesis in 3T3-L1 adipocytes, such as resveratrol and vitisin A. The results indicated that decrease in adipogenesis by resveratrol was associated with increase in the expression of Sirt1,which promotes fat mobilization by repressing PPARγ. However, vitisin A inhibits adipogenesis through p21- and Rb-dependent cell cycle arrest and consequent suppression of preadipocyte proliferation. Keywords: flavonoids, resveratrol, vitisin A, cell differentiation, adipogenesis. References 洪绣茹。2005。3T3-L1脂肪細胞培養及分化平台建立及其應用於活性藻類的篩選。國立中央大學生命科學研究所論文。桃園縣。 陳盈臻。2003。蔬果成分物質對3T3-L1脂肪細胞分化的影響。國立台灣大學園藝學研究所論文。台北市。 葉怡均。2004。共軛亞麻油酸對前脂肪細胞分化之影響。國立台灣海洋大學食品科學系碩士論文。基隆市。 張立昇。2004。桑色素（Morin）藉由細胞週期停滯及活化粒線體路徑誘導細胞凋亡進而抑制人