表观遗传与疾病.pptVIP

* * * * * The Strategy Purify Ajuba-associated protein complexes by large scale affinity chromatography combined with Mass spectrometry (MS) : stably express Flag-epitope tagged Ajuba in 293 cells, and the Ajuba associated protein complexes were immunoprecipitated with anti-Flag M2 beads. The components of the complexes were identified by MS. * Affinity purification of Ajuba-interacting proteins from 293 cells * Prmt5 is a type II Protein Arginine methyltransferase Type I: Prmt1, 2, 3, 4, and 6 Type II: Prmt5 * E-box Zn Zn Zn Zn Zn Zn Zn Zn Zn SNAG Ajuba LIM Snail preLIM PRMT5 MEP50 E-cadheri