过氧化氢参与水杨酸诱导的蚕豆气孔关闭过程观察-河南农业科学.PDF

DOI :10.15933/ki .1004 -3268.2008.03.002 河南农业科学 1 1 2 2 ＊ 何金环 , 王文静 , 王朋涛 ,宋纯鹏 (1. , 450011;2. , 47500 1) :利用表皮条生物分析、激光扫描共聚焦显微术、显微注射等技术, 研究观察了蚕豆气 关闭 过程, 结果表明,SA 浓度在 1 ～1000μmol/ L 时诱导的气 关闭具有可逆性, 而10-2 mol/ L 时导 致的气 关闭不可逆;20 U/mL 的过氧化氢酶(catalase, CAT )与SA 共同处理时可逆转SA 诱导 气 关闭作用的83%～90%。以H2 O2 荧光探针H2 DCFDA 结合激光扫描共聚焦显微术, 直接检 测保 卫细胞内H2 O2 的产生,结果表明, 与外源10-5 mol/L 的H2 O2 的处理结果类似。外施或通过 显微注射技术而在保 卫细胞内引入 100μmol/L 的SA 均可引起保 卫细胞内DCF 荧光快速增强。 在保卫细胞内显微注射CAT 可完全阻止SA 导致的DCF 荧光增强。表明SA 诱导的气 关闭可 能与H2 O2 的产生有关。 :蚕豆;过氧化氢;气 ;水杨酸 :Q945　　:A 　　:1004-3268(2008)03-0023-05 In ol ement of Hydrogen Peroxide in Stomatal Closure of Vicia f aba L.Induced by Salicylic A cid 1 1 2 2 ＊ HE Jin-huan , WANG Wen-jing ,WANG Peng-tao ,SONG Chun-peng (1.Department of Bioengineering,Zhengzhou College of Animal Husbandry and Engineering, Zhengzhou 450011, China;2.Department of Biology, H enan Uni ersity, Kaifeng 475001, China) Abstract:In the experiment, SA induced stomatal closure with a concentration-dependent man- ner,and the changes in stomata apertures w ere re ersible w hen the concentration of SA w as 1- 1000μmol/L,but irre ersible w hen the concentration of SA w as 10-2 mol/L.The treatment with SA and CA T of 20 U/mL re ersed the SA-induced stomatal closure by 83%-90%.The proto - plasts of guard cells in Vicia f aba L .w ere isolated by tw o-step enzyme digestion.We directly ex - 2 2 2 amined the production of H O by laser scanning confocal microscopy based on H DCFDA, similar -5 2 2 t