一株海洋放线菌的发酵培养及产物分析.pdfVIP

中文摘要 海洋放线菌是多种新型生物代谢物的来源。本实验对一株海洋放线菌链霉菌 属进行一段时间的发酵培养，继而对其粗品进行分离纯化等相关操作，并且对提 取到的次生代谢产物的结构进行有效的鉴定。首先对海洋放线菌链霉菌属进行发 酵，继而培养3个月。最后将其萃取，并采用硅胶柱层析法，凝胶柱层析法与反 相柱层析法以及薄层色谱法等技术手段对粗体物进行分离和纯化；最后通过质谱 以及核磁共振波谱对得到的单体化合物进行结构鉴定。本实验通过对一株海洋放 线菌链霉菌属进行发酵，继而对发酵提取物进行萃取，分离与纯化等操作，最后 经过色谱法对化合物的结构进行鉴定，最终得到了单体化合物，通过相关文献、 数据库的对照和参考，确定其化学式为C H NO ；名称为 3-正庚基-3-羟基 16 21 3 -1,2,3,4-四氢喹啉-2,4-二酮，其CAS编号为69808-30-6。 关键词：海洋放线菌；链霉菌属；次生代谢产物；分离与纯化；结构鉴定 I Abstract Marine actinomycetes is an ongoing source of novel bioactive metabolites. By liquid fermenting a marine actinomycete strain(Streptomycetes) for a period of time, and then followed by extraction，separation, purification, and conducting biological activity studies and structural identification of the Isolated secondary metabolites. First, the marine actinomycetes - Streptomyces (Streptomycetes) were subjected to liquid fermentation, then extraction, and separated and purified by Silica gel column chromatography ， Gel column chromatography ， Reverse phase column chromatography，andThin layer chromatography;Finally,the structure and chemical properties of the compounds were identified by mass spectrometryand Nuclear magnetic resonance . In this experiment, a marine actinomycete strain (Streptomycetes) was liquid fermented, then followed by extraction, isolation and purification of the fermentation extract, and finally through the chromatographic structure of its identification to obtain a compound.Its structure isC H NO ,it is 16 21 3 confirmed as 3-Heptyl-3-hydroxyquinolin-2,4-dione,its Cas Registry Number is 69808-30-6. Keywords:Marineactinomycetes ;Streptomycetes ;Secondarymetabolites ;Isolation andPurification ;Structuralidentification