一株黑蛋巢真菌的培养及其代谢产物研究.docxVIP

中文摘要 粪生黑蛋巢菌（Cyathus stercoreus (Schw.)de Toni）是四川黑蛋巢菌（Gyromitra xinjiangensis Cao, Fan et Liu sp. nov.）的一个分支，属于黑蛋巢菌属真菌（Cyathus）。以前的报道大多针对黑蛋巢菌属别的种真菌，而本文研究的这种粪生黑蛋巢菌次生代谢产物的相关研究报道较少，因此有很大的研究空间。本实验以这种粪生黑蛋巢菌为实验材料，对其进行固体发酵培养，提取得到丙酮粗提物，根据TLC薄层色谱跟踪分析，通过硅胶，凝胶，和反相柱层析等色谱分离方法，分离特异性化合物。通过质谱，1H-NMR、13C-NMR、DEPT等核磁共振这些波谱手段，对分离纯化得到的单体化合物，进行结构鉴定。最后我们从这种粪生黑蛋巢菌的丙酮粗提物中分离到2种化合物，通过波谱研究技术并参考相关文献和数据库，鉴定这两种化合物分别是：麦角甾醇和 2-non-1-enyl-1H-quinolin-4-one，这两种已知化合物在这种粪生黑蛋巢菌中是首次被分离得到。 关键词：粪生黑蛋巢真菌，代谢产物，培养提取，色谱分离，波谱鉴定 ABSTRACT Cyathus stercoreus (Schw.) De Toni is a branch of Gyromitra xinjiangensis Cao, Fan et Liu sp. Nov which belonging to the genus Cyathus. It looks like a black bird with black eggs nest ,and is widely distributed in our country. In this experiment, the fermented Cyathus was used as the experimental material, and the crude acetone extract from solid fermentation media was harvested for further isolation. Combined with TLC thin layer chromatography，the specific compounds can be traced and separated by the means of chromatographic methods such as silica gel column, reversed-phase Rp-C18 column and SephadexLH-20. The pure compounds were elucidated by means of spectroscopic techniques including mass spectrometry as well as nuclear magnetic resonance（1H-NMR、13C-NMR、DEPT）. Finally, we isolated two compounds from the crude acetone extract of the Cyathus. The compounds were identified by spectroscopy and the related literature and database. The two compounds were: ergosterol and 2-non-1 -enyl-1H-quinolin-4-one, and it is the first time that these two compounds have been isolated from this Cyathus. Key words: Cyathus, secondary metabolite, extraction and separation, structure identification  目录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc483984592 前 言 PAGEREF _Toc483984592 \h 1 HYPERLINK \l _Toc483984593 第一节 黑蛋巢菌及其次生代谢产物的研究现状 PAGEREF _Toc483984593 \h 1 HYPERLINK \l _Toc483984594 第二节 论文研究背景 PAGEREF _Toc483984594 \h 6 HYPERLINK \l _Toc483984595 第三节 论文研究思路与方法 PAGEREF _Toc483984595 \h 6 H