内生毛壳菌的发酵培养及次生代谢产物研究.docxVIP

PAGE \* MERGEFORMAT15 摘 要 真菌广泛存在于自然界中，是生物界中的一个很大的种群。到目前为止，世界上被人类记录的真菌约有12万种。真菌与人类生活密切相关，有些真菌有食用价值，有些真菌则具有药用价值，可以生产抗生素或治疗癌症等。植物的内生真菌种类繁多,分布广泛,可以产生各种活性天然产物，是一种丰富资源。目前为止，从植物的内生真菌中得到的次生代谢产物普遍具有抗炎、抗氧化、抗肿瘤和抗细胞毒等一系列活性。一些结构多样性次生代谢产物来源于毛壳属真菌。已经发现的某些次生代谢产物结构新颖并具有新的生物活性，例如抗炎、酶抑制活性、抗肿瘤、细胞毒性和抗菌活性等。 本文以天然产物化学的理论为依据，以微生物的发酵技术、有机溶剂的萃取技术以及柱层析色谱的分离技术为手段，将植物内生毛壳菌TY菌株进行扩大发酵培养，并对发酵液进行了分离提取及纯化。提取物的分离提纯主要通过三种色谱技术即Seghadex LH-20、反相色谱、薄层层析色谱相结合来达到目的。目前已经完成分离纯化工作，并且得到了一些纯度比较高的化合物样品，运用高分辨电子轰击质谱（EI-MS）技术结合氢谱（1H-NMR）技术对分离纯化所得到的物质进行结构鉴定，即可以推断出分离所得的化合物为何，甚至有可能进一步发现新的物质。 关键词：内生毛壳菌TY菌株；发酵；次生代谢产物；结构鉴定  Abstract There is a wide range of fungi in nature it belongs in the biosphere large populations.As of recent years there have been about 120,000 fungi discovered and recorded by humans around the world.Fungus plays an important role in human life and human beings have a very close relationship.Among them some fungi have edible value such as Pleurotus ostreatus and some fungi have medicinal value such as Ganoderma lucidum which can extract effective substances to treat cancer or produce antibiotics.Plant endophytic fungi a wide range of widely distributed can produce a variety of natural products is a rich resource.So far the secondary metabolites obtained from plant endophytic fungi generally have a series of activities such as anti-inflammatory anti-oxidation anti-tumor and anti-cytotoxicity.Some structural diversity of secondary metabolites derived from the genus Fungi.Some of the secondary metabolites that have been found are novel and have new biological activities such as anti-inflammatory enzymatic inhibitory activity antitumor cytotoxicity and antibacterial activity. The main basis of this article is the basic theory of natural product chemistry the use of organic solvent extraction technology microbial fermentation technology and chromatographic chromatography separation method and means to expand the fermentation of plant endophytic fungus TY strain,and then the fermentation broth was sep