基材弹性模量对兔纤维环源干细胞和骨髓间充质干细胞分化调控的比较分析-骨外科学专业论文.docxVIP

英文摘要 基材弹性模量对兔纤维环源干细胞和骨髓间充质干细胞分化调控的比较研究 The comparative study of modulation on the differentiation of rabbit annulus fibrosus-derived stem cells and rabbit bone marrow mesenchymal stem cells using scaffolds of various elastic moduli Abstract Objective: To isolate and culture rabbit annulus fibrosus-derived stem cells(AFSCs) and bone marrow mesenchymal stem cells(BMSCs) and manufacture PECUU scaffolds with different moduli. To make comparisons of differentiation ability of AFSCs and BMSCs into AF-like cells of radial regions of native annulus fibrosus using electrospun scaffolds of various moduli. Methods: We harvested the annulus fibrosus and bone marrow of the same six-month-old New Zealand white rabbit and isolated and cultured AFSCs and BMSCs. PECUU scaffolds of various moduli were fabricated using electrospinning technology. We planted AFSCs and TGF-β3-mediated BMSCs (tBMSCs) into scaffolds. The proliferation ability of both two types of stem cells in the scaffolds with MTS assay and the differentiation ability into AF-like cells of radial regions of native annulus fibrosus through RT-qPCR and CTFM technology were explored. Results: We successfully isolated and cultured AFSCs and BMSCs which expressed stem cell markers OCT-4, NS and SSEA-4 and were capable of induced adipogenesis, osteogenesis and chondrogenesis. PECUU scaffolds of various moduli with high porosity were fabricated. AFSCs and tBMSCs on the substrate appeared to proliferate well during culture. The expression of collagen-I in both tBMSCs and AFSCs increased with the increase of scaffold modulus, whereas the expression of collagen-II and aggrecan gene expression showed an opposite trend. Interestingly, the expression of collagen-I, collagen-II and aggrecan genes in tBMSCs on PECUU scaffolds were consistently higher than those in AFSCs regardless of scaffold modulus. In addition, the cell traction forces (CTFs) of both tBMSCs and AFSCs gradually decreased with the increase of scaffold II III III 基材弹性