利用基因芯片技术研究甘蓝型油菜油酸合成中差异表达基因-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2010, 36(6): 968−978 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.00968 利用基因芯片技术研究甘蓝型油菜油酸合成中差异表达基因 * 官 梅 李 栒 官春云 , 410128 : (71.71%)(55.6%) , 562 , 194 , 368 NM_100489 NM_130183 , , , Go 562 , , , / ACP Δ9 -(ADS1)Δ9 -2 (ADS2) ω-3 (fad3 ) : ; ; ; Differentially Expressed Genes in Oleic Acid Synthesis of Brassica napus by Detected Gene Chip * GUAN Mei, LI Xun , and GUAN Chun-Yun Oil Crops Research Institute, Hunan Agricultural University, Changsha 410128, China Abstract: Oil from rapeseed with high oleic oil content is insensitive to oxidation and long shelf life. In daily diet with high oleic acid oil can reduce low-density lipoprotein cholesterol levels in blood and prevent arteriosclerosis. In addition, high oleic acid content in edible oil is an important fat indicator. Oleic acid can be effectively converted to methyl ester, which is beneficial to produce biodiesel. Oleic acid content in conventional rapeseed is only 17%. However, oleic acid content in low erucic acid rape- seed reaches around 60%. Differential expressed genes of oleic acid have been found in the high oleic acid line (71.71%) and low oleic acid line (55.6%) of Brassica napus by gene chip technique. The results showed that there were 562 differential expressed genes relating to high and low oleic acid contents in rapeseed, among them 194 were up-regula