极低频电磁场对小鼠免疫功能和细胞增殖的影响-生物物理学专业论文.docxVIP

spleen index decreased, serum antibody(IgG、IgM、IgA) and complement(C3、C4) dropped, macrophage phagocytosis lower, mice in a state of low immune function. These show that the ELF-EMF can be an antagonistic role to Cy, mouse blood leukocyte number, the relative weight of the spleen, serum IgM and C3 content, macrophage phagocytosis of these immune indicators index rose; The ELF-EMF can also reinforce the normal mouse peritoneal macrophage phagocytosis, significantly increase serum IgG, IgM, C3 content of mouse, increase mouse spleen index. In addition, in the vitro experiment of time-dependence of the extremely low frequency electromagnetic radiation on activity of NK cells, we found that the activity of NK cells increased significantly which are exposed about 40-100min; with the increase of radiation time the activity of NK cells are increased and a peak occurs after the decline was parabolic. These results indicate that ELF-EMF can enhance the immunologic function and antagonize the inhibition of Cy on immunological function. This means that ELF-EMF can be an important means to improve the function of immune system in the future. 2. In the vitro experiment of influences of ELF-EMF on cell proliferation, we found that the C26 cells have the unremarkable colonies, low speed of growth and differentiation, decreased of density as well as shrinking of synapsis along with the exposed time of ELF-EMF. With the longer exposed time, the spleen cells grow sooner. In the meanwhile some cells become larger, more abundant cytoplasm, unit area increased the number of cells. The results of MTT colorimetric assay show that the proliferation of C26 cells which are exposed in the ELF-EMF could be inhibited significantly when compared with the control group (p 0.5).The inhibition effect is increased with the extension of the exposed time, enhanced at the beginning of a sharp increase, but the rate of increase is declined with the exposed time increasing farther. The extremely low fre