丙酸通路基因多态性与猪肉质及胴体性状的关联分析-遗传.PDF

HEREDITAS (Beijing) 2012 7 , 34(7): 872―878 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2012.00872 丙酸通路基因多态性与猪肉质及胴体性状的关联分析 杨帆, 王琼萍, 何侃, 王明辉, 潘玉春 , 200240 为了挖掘新的猪肉品质及胴体性状的候选基因, 揭示猪肉质及胴体性状的遗传机制, 文章将丙酸代谢通 路作为候选通路, 将通路内基因与猪肉质及胴体性状进行关联分析。实验采用 37 头三元杂交商品猪作为研究 对象, 首次针对丙酸通路中7 个基因的36 个SNP 位点利用SNaPshot 方法进行基因分型, 分别用最小二乘模型 及MB-MDR 模型与肉质及胴体性状进行关联分析。结果发现, 基因PCCB 、MUT 、MCEE 及ACSS2 上的4 个SNP 位点分别与肌内脂肪含量、背膘厚等性状显著相关(P0.05), ACSS2 与猪脂肪含量显著相关; MCEE 及MUT 与猪的 背膘厚显著相关; PCCB 基因与脂重显著相关。通过MB-MDR 方法检测到多个SNP 位点具有互作效应, 并与背膘 厚、水分含量、脂肪含量显著相关(P0.05) 。另外, 丙酸代谢通路中的基因间的互作效应对猪肉品质有显著影响。 丙酸代谢通路; 多态性; 肉质品质; 胴体品质; SNaPshot Association between gene polymorphisms of propanoate metabolism pathway and meat quality as well as carcass traits in pigs YANG Fan, WANG Qiong-Ping, HE Kan, WANG Ming-Hui, PAN Yu-Chun School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China Abstract: In order to gain more extensive insight into detailed genetic control mechanisms of porcine meat quality and mine novel candidate genes, this study focused on the relationship between the genes of propanpate metabolism pathway and porcine meat quality as well as carcass traits based on the candidate gene set approach. Thirty-seven DLY pigs were tested in this study. A total of 36 SNPs within 7 candidate genes of propanoate metabolism pathway were genotyped and association analysis was conducted via Least Squares method, Multivariate multiple regression model, and a model-based multifactor dimensionality reduction method (MB-MDR). As a result, four SNPs in genes PCCB, MUT , MCEE , and ACSS1 were significantly associated with DLY pig meat quality or carcass traits (P0.05).