甲锭丙二胺的合成方法研究-生物化工专业论文.docx

⑧：慨缏淅江丈翱埘究生毕业敞 ⑧：慨缏淅江丈翱埘究生毕业敞 甲锭丙：腔的合成施研究 七步合成反应减少到六步，节省了合成成本。减少了处理时间，使反应更加符合 实际开发的需要。 本论文所确定的合成工艺和选择的分离提纯方法，具有很好的重现性、稳定 性和可靠性。利用该合成工艺和分离工艺，在实验室，积累了一定量各步中间体 化合物，得到了5克的目标化合物，六步反应总收率为62．14％．实验总收率为 48．83％，甲锭丙二胺纯度为97．18％．该法适宜于较大规模制备甲锭丙二胺。 关键词t甲锭、甲锭丙二胺、MPE、合成、分离、工艺条件、表征、红外光谱、 ESI质谱、核磁共振、商效液相色谱、气质联用 Il ⑧ ⑧ 一煳文 甲锭丙二睦的音成方法研究 ABSTRACT Methidinmpropylamine is a key compotmd in synthesis ofmethidiumpropyla- mine-EDTA(MeE)that is used widely in the filed ofmedicine，biology and alh日 researches as DNA incising∞lufiom hthedissertation,theimportanceofthemethidiumpropylaminc astheprecursor ofDNA incising solution WaS described．Also，the ways ofsynthesis ofmcthidium- pmpylamine,the experimental foundation for the application and development of MPE were reviewⅨl in the dissertation．ARor the rationality mad feasthifity ofthe r印。删approachs being mmlyze l,a way to synthe∞methidiumpropylamine from cheap and available raw materials WflS explored．孵蹈reaction and separation condisfions being optimized，methidiumpropylamine was prepared succcssifully throu曲six steps．It turned out that the six-step reaction procedure gave sarisfy／ng y萌ld． Tk synthesis ofme吐ddillIl叩唧ylalnim used in the dissertation W3$ characterized wim good repeatabifity,stability and d印emdability．The p∞cedlm of anythasis ofmetlfidiumpropylamine devdopcd in the dissertation Was simplc tO operate and the quality ofthe product couId be controlled witIl ease：．Under the reaction and separation conditions detcmdned in the work,methidimnpropylamine was prepared with 48．83％oftotal yield and 97．18％ofpurlty． Themain resultsandinnovationsachievedinthework辩asfollowing． (1)．Reaction conditions ofeach step w日e optimized and the key factOrs that affects the reactions WCCe studied，The yields for nitration,amidation,cyclization, m础y1撕on，deoxidization and antidation reactions WOle 90．24％，86．61％，99．12％。 98．34％，89．96％and 91．12％respectively．The tOtal yield ofmethidiumpropylamine synthesiswas48 83％with97．18％purity,whichWaShightlthanthat repo