基于PLSR分析当归挥发油分子蒸馏馏分中化学成分与抗-环球中医药.PDF

环球中医药2015年10月第8卷第10期摇 Global Traditional Chinese Medicine,October2015,Vol郾8,No郾10 摇1153 ·论著 · 基于PLSR分析当归挥发油分子蒸馏馏分 中化学成分与抗炎作用的相关性 张庆摇 茹庆国摇 林红梅摇 刘艳摇 康倩摇 李辉摇 吴清 揖摘要铱摇 目的摇 研究当归挥发油分子蒸馏馏分的化学成分与其抗炎作用的相关性。 方法摇 用 分子蒸馏设备对当归挥发油进行分馏,气质联用色谱(gas chromatography鄄mass spectroscopy,GC鄄MS) 表征所得馏分的化学组成;用脂多糖(lipopolysaccharide,LPS)诱导 RAW264.7 细胞作为炎症模型, 评价各馏分的细胞毒作用和抗炎作用;用偏最小二乘法回归分析(partial least squares regression, PLSR)分析馏分中化学成分与抗炎作用的相关性。 结果摇 当归挥发油经分子蒸馏后得到6个馏分, 且各馏分均能抑制LPS诱导的RAW264.7 细胞产生一氧化氮,表现出抗炎作用;PLSR结果显示建 立的回归模型合理,馏分中的十九烷、2,2鄄二甲基鄄1鄄苯基鄄1鄄丙醇、Z鄄藁本内酯、E鄄藁本内酯、十六烷、 阿魏酸、十三酸、亚油酸、油酸、5,8,11鄄十七碳三炔酸甲酯与抗炎作用呈正相关。 结论摇 本研究为当 归及其他中药挥发油的进一步开发利用及中药挥发油的质量控制提供了实验数据。 揖关键词铱摇 当归挥发油;摇 分子蒸馏;摇 气质联用色谱;摇 抗炎;摇 偏最小二乘法 揖中图分类号铱摇 R285.5摇 揖文献标识码铱摇 A摇 doi:10郾3969/ j郾issn郾1674鄄1749郾2015郾10郾001 Correlation between chemical constituents and anti鄄inflammatory activity of distillates separated from essential oil of Angelica Sinensis Radix by molecular distillation based on PLSR摇 ZHANG Qing,RUQing鄄guo,LINHong鄄mei,etal. Schoolof ChineseMateriaMedica,Beijing University of Chinese Medicine,Beijing 100102,China Corresponding author:WUQing,E鄄mail:qwu@ 揖Abstract铱摇 Objective摇 To investigate the correlation between the chemical constituents and anti鄄 inflammatory effect of distillates separated from the essential oil of Angelica Sinensis Radix by molecular distillation.Methods摇 The distillates were separated from the essential oil of Angelica Sinensis Radix by molecular distillation, and the chemical constituents of distillates were analyzed by GC鄄MS; lipopolysaccharide (LPS) 鄄 induced RAW264.7 cells as a model of inflammation to evaluate the cell toxicity and anti鄄inflammatory effects of distillates;partial least squares regression (PLSR) was evaluated to analysisthecorrelationbetweenchemicalconstituentsandanti鄄inflammatoryeffect. Results摇 6fractions obtained